Dental crown biomineralization during its histogenesis

Oleksij P. Kostyrenko, Nataliia I. Vynnyk, Mykhailo M. Koptev, Petro A. Hasiuk, Maksym I. Skrypnyk, Alevtyna M. Bilous, Serhii A. Proskurnya

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6 Citations (Scopus)

Abstract

The aim: The paper was aimed at the study of the biomineralization processes of a permanent dental crown in the postnatal period of histogenesis.
Materials and methods: The study involved 30 culled puppies aged 30-40 days. To study the histogenesis of the germs of the permanent tooth from the cuticular epithelium in the postnatal period microscopic, electron microscopic, immunohistochemical methods of study have been used.
Results: The studies show that in the postnatal period, the maturation of the germ of a permanent tooth starts with the synthesis of cells of the cuticular epithelium of the organic stroma, capable of subsequent mineralization. Differentiation of the proameloblasts, located on the surface of the dental papillary mesenchyma, at the early stages of histogenesis, is strongly associated with the appearance of a specific protein taftelin. Origination of secretory ameloblasts, which produce the protein enamelin, triggers the process of secondary biomineralization of the enamel. The terminal processes of the secretory ameloblasts produce the protein in the form of layers that overlap each other at a certain angle. Such layering of the structures of enamel and dentin contributes to the S-shaped maturation of the hard tooth tissues, strengthening them considerably. At the follicle stage, maturation of the dental crown, coated with cuticular epithelium, occurs. Invaginations of the cuticular epithelium form a characteristic topography of the dental crown, and enamel projections are further formed by the ameloblasts. Epitheliocytes of the inner layer of the enamel organ have desmosomal connections that allow the filtration of the salivary fluid at the stage of the enamel trophism.
Conclusions: The findings of the study suggest that, normally, due to the cuticular epithelium, filtration of the salivary fluid occurs with protein deposition on it and subsequent infiltration of the calcium salts into the subjacent enamel.
Original languageEnglish
Pages (from-to)2612-2616
Number of pages5
JournalWiadomosci Lekarskie
Volume73
Issue number12 Part 1
DOIs
Publication statusPublished - Dec 2020
Externally publishedYes

Bibliographical note

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Keywords

  • histogenesis
  • enamel
  • dentine
  • biomineralization
  • cuticular epithelium

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