Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP

Shilun Feng, Tao Dong*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

2 Citations (Scopus)

Abstract

A novel multifunctional two-sides silicon lab-on-chip (LOC) was designed and fabricated for providing a high-efficient long-term continuous analysis platform of measuring the concentration of adenosine 5'-triphosphate (ATP) based on bioluminescence. This lab-on-chip mainly consists of Y shape internal ribbed micro mixers, T-junction droplet generators, different kinds of spiral time delay channels (TD-Cs) and the chambers. Enzyme solutions and ATP were separately injected into the chip at different flow rates through 3 inlet ports. Due to the advantage of Segmented Flow Analysis (SFA), it was introduced and applied with the T-junction droplet generators in this paper, and thus oxygen was injected into the central of the bottom surface through another main inlet periodically. Numerical investigations prove that combing with SFA and T-junction droplet generator is stable to transport the sample; micro mixer can achieve an outstanding mixing efficiency in a small space; the detection of ATP standard solution at optimal conditions was successful, keeping a good linearity between the concentration with the intensity of light during the range from 10-5 mol /ml to 10-9 mol/ml.

Original languageEnglish
Title of host publicationMeMeA 2014
Subtitle of host publication2014 IEEE International Symposium on Medical Measurements and Applications : proceedings
Place of PublicationPiscataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Number of pages4
ISBN (Print)9781479929207
DOIs
Publication statusPublished - 2014
Externally publishedYes
Event9th IEEE International Symposium on Medical Measurements and Applications, IEEE MeMeA 2014 - Lisbon, Portugal
Duration: 11 Jun 201412 Jun 2014

Other

Other9th IEEE International Symposium on Medical Measurements and Applications, IEEE MeMeA 2014
CountryPortugal
CityLisbon
Period11/06/1412/06/14

Keywords

  • lab-on-chip
  • internal ribbed micro mixer
  • Tjunction droplet generator
  • adenosine 5'-triphosphate (ATP)

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