Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP

Shilun Feng; Tao Dong

doi:10.1109/MeMeA.2014.6860076

Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP

Shilun Feng, Tao Dong^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

2 Citations (Scopus)

Abstract

A novel multifunctional two-sides silicon lab-on-chip (LOC) was designed and fabricated for providing a high-efficient long-term continuous analysis platform of measuring the concentration of adenosine 5'-triphosphate (ATP) based on bioluminescence. This lab-on-chip mainly consists of Y shape internal ribbed micro mixers, T-junction droplet generators, different kinds of spiral time delay channels (TD-Cs) and the chambers. Enzyme solutions and ATP were separately injected into the chip at different flow rates through 3 inlet ports. Due to the advantage of Segmented Flow Analysis (SFA), it was introduced and applied with the T-junction droplet generators in this paper, and thus oxygen was injected into the central of the bottom surface through another main inlet periodically. Numerical investigations prove that combing with SFA and T-junction droplet generator is stable to transport the sample; micro mixer can achieve an outstanding mixing efficiency in a small space; the detection of ATP standard solution at optimal conditions was successful, keeping a good linearity between the concentration with the intensity of light during the range from 10^-5 mol /ml to 10^-9 mol/ml.

Original language	English
Title of host publication	MeMeA 2014
Subtitle of host publication	2014 IEEE International Symposium on Medical Measurements and Applications : proceedings
Place of Publication	Piscataway, NJ
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Number of pages	4
ISBN (Print)	9781479929207
DOIs	https://doi.org/10.1109/MeMeA.2014.6860076
Publication status	Published - 2014
Externally published	Yes
Event	9th IEEE International Symposium on Medical Measurements and Applications, IEEE MeMeA 2014 - Lisbon, Portugal Duration: 11 Jun 2014 → 12 Jun 2014

Other

Other	9th IEEE International Symposium on Medical Measurements and Applications, IEEE MeMeA 2014
Country/Territory	Portugal
City	Lisbon
Period	11/06/14 → 12/06/14

Keywords

lab-on-chip
internal ribbed micro mixer
Tjunction droplet generator
adenosine 5'-triphosphate (ATP)

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10.1109/MeMeA.2014.6860076

Cite this

@inproceedings{bd68962b071148d9b6c237a44ffc0b6b,

title = "Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP",

abstract = "A novel multifunctional two-sides silicon lab-on-chip (LOC) was designed and fabricated for providing a high-efficient long-term continuous analysis platform of measuring the concentration of adenosine 5'-triphosphate (ATP) based on bioluminescence. This lab-on-chip mainly consists of Y shape internal ribbed micro mixers, T-junction droplet generators, different kinds of spiral time delay channels (TD-Cs) and the chambers. Enzyme solutions and ATP were separately injected into the chip at different flow rates through 3 inlet ports. Due to the advantage of Segmented Flow Analysis (SFA), it was introduced and applied with the T-junction droplet generators in this paper, and thus oxygen was injected into the central of the bottom surface through another main inlet periodically. Numerical investigations prove that combing with SFA and T-junction droplet generator is stable to transport the sample; micro mixer can achieve an outstanding mixing efficiency in a small space; the detection of ATP standard solution at optimal conditions was successful, keeping a good linearity between the concentration with the intensity of light during the range from 10-5 mol /ml to 10-9 mol/ml.",

keywords = "lab-on-chip, internal ribbed micro mixer, Tjunction droplet generator, adenosine 5'-triphosphate (ATP)",

author = "Shilun Feng and Tao Dong",

year = "2014",

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language = "English",

isbn = "9781479929207",

booktitle = "MeMeA 2014",

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address = "United States",

note = "9th IEEE International Symposium on Medical Measurements and Applications, IEEE MeMeA 2014 ; Conference date: 11-06-2014 Through 12-06-2014",

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Feng, S & Dong, T 2014, Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP. in MeMeA 2014: 2014 IEEE International Symposium on Medical Measurements and Applications : proceedings. Institute of Electrical and Electronics Engineers (IEEE), Piscataway, NJ, 9th IEEE International Symposium on Medical Measurements and Applications, IEEE MeMeA 2014, Lisbon, Portugal, 11/06/14. https://doi.org/10.1109/MeMeA.2014.6860076

Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP. / Feng, Shilun; Dong, Tao.
MeMeA 2014: 2014 IEEE International Symposium on Medical Measurements and Applications : proceedings. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE), 2014.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP

AU - Feng, Shilun

AU - Dong, Tao

PY - 2014

Y1 - 2014

N2 - A novel multifunctional two-sides silicon lab-on-chip (LOC) was designed and fabricated for providing a high-efficient long-term continuous analysis platform of measuring the concentration of adenosine 5'-triphosphate (ATP) based on bioluminescence. This lab-on-chip mainly consists of Y shape internal ribbed micro mixers, T-junction droplet generators, different kinds of spiral time delay channels (TD-Cs) and the chambers. Enzyme solutions and ATP were separately injected into the chip at different flow rates through 3 inlet ports. Due to the advantage of Segmented Flow Analysis (SFA), it was introduced and applied with the T-junction droplet generators in this paper, and thus oxygen was injected into the central of the bottom surface through another main inlet periodically. Numerical investigations prove that combing with SFA and T-junction droplet generator is stable to transport the sample; micro mixer can achieve an outstanding mixing efficiency in a small space; the detection of ATP standard solution at optimal conditions was successful, keeping a good linearity between the concentration with the intensity of light during the range from 10-5 mol /ml to 10-9 mol/ml.

AB - A novel multifunctional two-sides silicon lab-on-chip (LOC) was designed and fabricated for providing a high-efficient long-term continuous analysis platform of measuring the concentration of adenosine 5'-triphosphate (ATP) based on bioluminescence. This lab-on-chip mainly consists of Y shape internal ribbed micro mixers, T-junction droplet generators, different kinds of spiral time delay channels (TD-Cs) and the chambers. Enzyme solutions and ATP were separately injected into the chip at different flow rates through 3 inlet ports. Due to the advantage of Segmented Flow Analysis (SFA), it was introduced and applied with the T-junction droplet generators in this paper, and thus oxygen was injected into the central of the bottom surface through another main inlet periodically. Numerical investigations prove that combing with SFA and T-junction droplet generator is stable to transport the sample; micro mixer can achieve an outstanding mixing efficiency in a small space; the detection of ATP standard solution at optimal conditions was successful, keeping a good linearity between the concentration with the intensity of light during the range from 10-5 mol /ml to 10-9 mol/ml.

KW - lab-on-chip

KW - internal ribbed micro mixer

KW - Tjunction droplet generator

KW - adenosine 5'-triphosphate (ATP)

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DO - 10.1109/MeMeA.2014.6860076

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SN - 9781479929207

BT - MeMeA 2014

PB - Institute of Electrical and Electronics Engineers (IEEE)

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Y2 - 11 June 2014 through 12 June 2014

ER -

Design and characterization of a lab-on-chip for continuous bioluminescent measurements of ATP

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