Design and non-covalent DNA binding of platinum (II) metallacalix[4]arenes

Miguel A. Galindo, David Olea, M. Angustias Romero, Julio Gómez, Pedro Del Castillo, Michael J. Hannon*, Alison Rodger, Félix Zamora, Jorge A R Navarro

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

A set of cyclic tetranuclear complexes of the metallacalix[4]arene type with formula [{Pt(en)(L)}4]4+ (en = ethylenediamine; 2: LH = 5-chloro-2-hydroxypyrimidine (5-Cl-Hpymo); 3: LH=5-bromo-2-hydroxypyrimidine (5-Br-Hpymo); 4: LH = 5-iodo-2-hydroxypyrimidine (5-I-Hpymo)) have been obtained from the reaction between cis-protected square-planar [Pt(en)(H 2O)2]2+ metal entities and LH in aqueous media. Additionally, the binding properties of 2, 3, 4 and their congener [{Pt(en)(L)}4]4+ (1: LH = 2-hydroxypyrimidine (Hpymo)) with calf thymus-DNA (ct-DNA) have been studied by using different techniques including circular and linear dichroism (CD and LD, respectively) and UV-visible absorbance spectroscopies, gel electrophoresis, fluorescence competitive-binding studies and atomic force microscopy (AFM). The results are consistent with significant non-covalent interactions taking place between the polynuclear cyclic species and ct-DNA. Moreover, gel electrophoresis, linear dichroism titrations and AFM images of ct-DNA with metallacalixarenes show ct-DNA coiling at low metallacalixarene concentrations and upon subsequent increments in metallacalixarene concentration ct-DNA can be seen to uncoil with concomitant formation of long and inflexible ct-DNA structures.

Original languageEnglish
Pages (from-to)5075-5081
Number of pages7
JournalChemistry - A European Journal
Volume13
Issue number18
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Atomic force microscopy
  • Binding studies
  • DNA
  • Metal-lacalixarenes
  • Platinum

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