The design of multi-band bandpass filters (BPFs) employing stub loaded stepped-impedance resonator with defected microstrip structure (SL-SIR-DMS) is presented in this study for the first time. The proposed SL-SIR-DMS is created by embedding DMS on the low-impedance line of the SL-SIR. It is found that different defected structures can lead to different frequency responses. In addition, by using DMS, tri-band and even quad-band responses can be easily achieved without increasing the resonator size. As verification, one tri-band SL-SIR-DMS and one quad-band SL-SIR-DMS are designed and analysed using even/odd-mode method. Subsequently, one tri-band BPF and one quad-band BPF have been developed with pseudo-interdigital coupling to realise good out-of-band performance. The predicted results are compared with measured ones and good agreement is achieved. Compared with BPFs using only SL-SIR, the proposed filters with DMS are more compact due to the slow-wave characteristic. Compared with the BPFs using defected ground structure to improve the stopband performance, the proposed ones can realise comparable wide stopbands but maintaining the signal integrity on the ground plane for packaging purpose.