Design, synthesis, characterization, and in vitro evaluation of a new cross-linked hyaluronic acid for pharmaceutical and cosmetic applications

Sabrina Sciabica, Giovanni Tafuro, Alessandra Semenzato, Daniela Traini, Dina M. Silva, Larissa Gomes Dos Reis, Luisa Canilli, Massimo Terno, Elisa Durini, Silvia Vertuani, Anna Baldisserotto, Stefano Manfredini

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
82 Downloads (Pure)

Abstract

Hyaluronic acid (HA), an excellent biomaterial with unique bio properties, is currently one of the most interesting polymers for many biomedical and cosmetic applications. However, several of its potential benefits are limited as it is rapidly degraded by hyaluronidase enzymes. To improve the half-life and consequently increase performance, native HA has been modified through cross-linking reactions with a natural and biocompatible amino acid, Ornithine, to overcome the potential toxicity commonly associated with traditional linkers. 2-chloro-dimethoxy-1,3,5-triazine/4-methylmorpholine (CDMT/NMM) was used as an activating agent. The new product (HA-Orn) was extensively characterized to confirm the chemical modification, and rheological analysis showed a gel-like profile. In vitro degradation experiments showed an improved resistance profile against enzymatic digestions. Furthermore, in vitro cytotoxicity studies were performed on lung cell lines (Calu-3 and H441), which showed no cytotoxicity.

Original languageEnglish
Article number1672
Pages (from-to)1-17
Number of pages17
JournalPharmaceutics
Volume13
Issue number10
DOIs
Publication statusPublished - 13 Oct 2021

Bibliographical note

Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • hyaluronic acid
  • cross-linking
  • biocompatibility

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