Detection and density estimation of goblet cells in confocal endoscopy for the evaluation of celiac disease

D. Boschetto, H. Mirzaei, R. W L Leong, E. Grisan

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

4 Citations (Scopus)

Abstract

Celiac Disease (CD) is an immune-mediated enteropathy, diagnosed in the clinical practice by intestinal biopsy and the concomitant presence of a positive celiac serology. Confocal Laser Endomicroscopy (CLE) allows skilled and trained experts to potentially perform in vivo virtual histology of small-bowel mucosa. In particular, it allows the qualitative evaluation of mucosa alteration such as a decrease in goblet cells density, presence of villous atrophy or crypt hypertrophy. We present a semi-automatic computer-based method for the detection of goblet cells from confocal endoscopy images, whose density changes in case of pathological tissue. After a manual selection of a suitable region of interest, the candidate columnar and goblet cells' centers are first detected and the cellular architecture is estimated from their position using a Voronoi diagram. The region within each Voronoi cell is then analyzed and classified as goblet cell or other. The results suggest that our method is able to detect and label goblet cells immersed in a columnar epithelium in a fast, reliable and automatic way. Accepting 0.44 false positives per image, we obtain a sensitivity value of 90.3%. Furthermore, estimated and real goblet cell densities are comparable (error: 9.7 ± 16.9%, correlation: 87.2%, R2 = 76%).

Original languageEnglish
Title of host publicationEMBC 2015
Subtitle of host publicationProceedings of the 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Place of PublicationNew York
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages6248-6251
Number of pages4
ISBN (Electronic)9781424492718, 9781424492701
DOIs
Publication statusPublished - 4 Nov 2015
Externally publishedYes
Event37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015 - Milan, Italy
Duration: 25 Aug 201529 Aug 2015

Other

Other37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
CountryItaly
CityMilan
Period25/08/1529/08/15

Keywords

  • celiac disease
  • computer-aided diagnosis
  • confocal endomicroscopy
  • goblet cells

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