Detection of Chlamydophila pneumoniae DNA in peripheral blood mononuclear cells of blood donors in the north-east of Italy

Mario Rassu; Federico M. Lauro; Stefania Cazzavillan; Emanuela Bonoldi; Maurizio Belloni; Maria C. Bettini; Alberto Pilotto; Carlo Mengoli; Antonella Peron; Renato Zambello; Mariuccia Scagnelli; Giulio Bertoloni

Detection of Chlamydophila pneumoniae DNA in peripheral blood mononuclear cells of blood donors in the north-east of Italy

Mario Rassu^*, Federico M. Lauro, Stefania Cazzavillan, Emanuela Bonoldi, Maurizio Belloni, Maria C. Bettini, Alberto Pilotto, Carlo Mengoli, Antonella Peron, Renato Zambello, Mariuccia Scagnelli, Giulio Bertoloni

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Recent studies have implicated Chlamydia pneumoniae (now Chlamydophila pneumoniae) in the pathogenesis of atherosclerosis and demonstrated its presence within human peripheral blood mononuclear cells (PBMCs). In this study the presence of C. pneumoniae DNA was assessed, using nested PCR, in PBMCs from 169 active blood donors as a function of age, of specific antibodies and C-reactive protein. The results obtained demonstrated a high degree of global positivity (46.15%), which was higher in females (52%) than in males (43.7%). Seroepidemiological studies showed a high percentage of positivity both in subjects positive by PCR (65.91%) and negative by PCR (71.74%). The clinical implication of such finding are under study.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Medical Microbiology and Immunology
Volume	190
Issue number	3
Publication status	Published - 2001
Externally published	Yes

Keywords

Antibodies
Blood donors
Chlamydophila pneumoniae
Peripheral blood mononuclear cells
Polymerase chain reaction

Cite this

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title = "Detection of Chlamydophila pneumoniae DNA in peripheral blood mononuclear cells of blood donors in the north-east of Italy",

abstract = "Recent studies have implicated Chlamydia pneumoniae (now Chlamydophila pneumoniae) in the pathogenesis of atherosclerosis and demonstrated its presence within human peripheral blood mononuclear cells (PBMCs). In this study the presence of C. pneumoniae DNA was assessed, using nested PCR, in PBMCs from 169 active blood donors as a function of age, of specific antibodies and C-reactive protein. The results obtained demonstrated a high degree of global positivity (46.15%), which was higher in females (52%) than in males (43.7%). Seroepidemiological studies showed a high percentage of positivity both in subjects positive by PCR (65.91%) and negative by PCR (71.74%). The clinical implication of such finding are under study.",

keywords = "Antibodies, Blood donors, Chlamydophila pneumoniae, Peripheral blood mononuclear cells, Polymerase chain reaction",

author = "Mario Rassu and Lauro, {Federico M.} and Stefania Cazzavillan and Emanuela Bonoldi and Maurizio Belloni and Bettini, {Maria C.} and Alberto Pilotto and Carlo Mengoli and Antonella Peron and Renato Zambello and Mariuccia Scagnelli and Giulio Bertoloni",

year = "2001",

language = "English",

volume = "190",

pages = "139--144",

journal = "Medical Microbiology and Immunology",

issn = "0300-8584",

publisher = "Springer, Springer Nature",

number = "3",

}

TY - JOUR

T1 - Detection of Chlamydophila pneumoniae DNA in peripheral blood mononuclear cells of blood donors in the north-east of Italy

AU - Rassu, Mario

AU - Lauro, Federico M.

AU - Cazzavillan, Stefania

AU - Bonoldi, Emanuela

AU - Belloni, Maurizio

AU - Bettini, Maria C.

AU - Pilotto, Alberto

AU - Mengoli, Carlo

AU - Peron, Antonella

AU - Zambello, Renato

AU - Scagnelli, Mariuccia

AU - Bertoloni, Giulio

PY - 2001

Y1 - 2001

N2 - Recent studies have implicated Chlamydia pneumoniae (now Chlamydophila pneumoniae) in the pathogenesis of atherosclerosis and demonstrated its presence within human peripheral blood mononuclear cells (PBMCs). In this study the presence of C. pneumoniae DNA was assessed, using nested PCR, in PBMCs from 169 active blood donors as a function of age, of specific antibodies and C-reactive protein. The results obtained demonstrated a high degree of global positivity (46.15%), which was higher in females (52%) than in males (43.7%). Seroepidemiological studies showed a high percentage of positivity both in subjects positive by PCR (65.91%) and negative by PCR (71.74%). The clinical implication of such finding are under study.

AB - Recent studies have implicated Chlamydia pneumoniae (now Chlamydophila pneumoniae) in the pathogenesis of atherosclerosis and demonstrated its presence within human peripheral blood mononuclear cells (PBMCs). In this study the presence of C. pneumoniae DNA was assessed, using nested PCR, in PBMCs from 169 active blood donors as a function of age, of specific antibodies and C-reactive protein. The results obtained demonstrated a high degree of global positivity (46.15%), which was higher in females (52%) than in males (43.7%). Seroepidemiological studies showed a high percentage of positivity both in subjects positive by PCR (65.91%) and negative by PCR (71.74%). The clinical implication of such finding are under study.

KW - Antibodies

KW - Blood donors

KW - Chlamydophila pneumoniae

KW - Peripheral blood mononuclear cells

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0035212222&partnerID=8YFLogxK

M3 - Article

C2 - 11827203

AN - SCOPUS:0035212222

SN - 0300-8584

VL - 190

SP - 139

EP - 144

JO - Medical Microbiology and Immunology

JF - Medical Microbiology and Immunology

IS - 3

ER -

Detection of Chlamydophila pneumoniae DNA in peripheral blood mononuclear cells of blood donors in the north-east of Italy

Abstract

Keywords

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