Detection of cytoplasmic and nuclear localization of adenomatous polyposis coli (APC) protein in cells

Mariana Brocardo*, Beric R. Henderson

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

The adenomatous polyposis coli (APC) tumour suppressor gene is mutated in the majority of colon cancers. APC is a multi-domain protein whose distribution at different subcellular locations correlates with unique cellular processes. Our laboratory has focused on the link between APC subcellular location and function, and has characterized pathways for the trafficking of APC both into and out of the nucleus. Antibody specificity is an important factor in the determination of APC localization, and in this chapter we outline a strategy for the unambiguous detection of APC using a combination of biochemical and cell biology approaches.

Original languageEnglish
Title of host publicationWnt signaling
Subtitle of host publicationVolume 1, Pathway Methods and Mammalian Models
EditorsElizabeth Vincan
Place of PublicationNew York
PublisherHumana Press
Pages77-89
Number of pages13
ISBN (Print)9781588299123
DOIs
Publication statusPublished - 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume468
ISSN (Print)10643745

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