Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer

Philip Butterworth; Henrique T. M. C. M. Baltar; Martin Kratzmeier; Ewa M. Goldys

doi:10.1007/978-1-61779-806-1_13

Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer

Philip Butterworth, Henrique T. M. C. M. Baltar, Martin Kratzmeier, Ewa M. Goldys^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Citations (Scopus)

Abstract

Bead assays are an emerging microbial detection technology with the capability for rapid detection of extremely low levels of viable pathogens. Such technologies are of high value in clinical settings and in the food industry. Here, we perform a bead assay for extracted 16S rRNA from Escherichia coli (strain K12) with the flow cytometry readout on a 2100 Bioanalyzer, a highly accurate, small-scale flow cytometer system.

Original language	English
Title of host publication	Spectroscopic Methods of Analysis
Subtitle of host publication	Methods and Protocols
Editors	Wlodek M. Bujalowski
Place of Publication	New York
Publisher	Humana Press Inc.
Pages	253-262
Number of pages	10
ISBN (Electronic)	9781617798061
ISBN (Print)	9781617798054
DOIs	https://doi.org/10.1007/978-1-61779-806-1_13
Publication status	Published - 2012

Publication series

Name	Methods in Molecular Biology
Volume	875
ISSN (Print)	1064-3745

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Butterworth, P., Baltar, H. T. M. C. M., Kratzmeier, M., & Goldys, E. M. (2012). Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer. In W. M. Bujalowski (Ed.), Spectroscopic Methods of Analysis: Methods and Protocols (pp. 253-262). (Methods in Molecular Biology; Vol. 875). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-806-1_13

Butterworth, Philip ; Baltar, Henrique T. M. C. M. ; Kratzmeier, Martin et al. / Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer. Spectroscopic Methods of Analysis: Methods and Protocols. editor / Wlodek M. Bujalowski. New York : Humana Press Inc., 2012. pp. 253-262 (Methods in Molecular Biology).

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abstract = "Bead assays are an emerging microbial detection technology with the capability for rapid detection of extremely low levels of viable pathogens. Such technologies are of high value in clinical settings and in the food industry. Here, we perform a bead assay for extracted 16S rRNA from Escherichia coli (strain K12) with the flow cytometry readout on a 2100 Bioanalyzer, a highly accurate, small-scale flow cytometer system.",

author = "Philip Butterworth and Baltar, {Henrique T. M. C. M.} and Martin Kratzmeier and Goldys, {Ewa M.}",

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Butterworth, P, Baltar, HTMCM, Kratzmeier, M & Goldys, EM 2012, Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer. in WM Bujalowski (ed.), Spectroscopic Methods of Analysis: Methods and Protocols. Methods in Molecular Biology, vol. 875, Humana Press Inc., New York, pp. 253-262. https://doi.org/10.1007/978-1-61779-806-1_13

Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer. / Butterworth, Philip; Baltar, Henrique T. M. C. M.; Kratzmeier, Martin et al.

Spectroscopic Methods of Analysis: Methods and Protocols. ed. / Wlodek M. Bujalowski. New York : Humana Press Inc., 2012. p. 253-262 (Methods in Molecular Biology; Vol. 875).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T1 - Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer

AU - Butterworth, Philip

AU - Baltar, Henrique T. M. C. M.

AU - Kratzmeier, Martin

AU - Goldys, Ewa M.

PY - 2012

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AB - Bead assays are an emerging microbial detection technology with the capability for rapid detection of extremely low levels of viable pathogens. Such technologies are of high value in clinical settings and in the food industry. Here, we perform a bead assay for extracted 16S rRNA from Escherichia coli (strain K12) with the flow cytometry readout on a 2100 Bioanalyzer, a highly accurate, small-scale flow cytometer system.

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A2 - Bujalowski, Wlodek M.

PB - Humana Press Inc.

CY - New York

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Butterworth P, Baltar HTMCM, Kratzmeier M, Goldys EM. Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer. In Bujalowski WM, editor, Spectroscopic Methods of Analysis: Methods and Protocols. New York: Humana Press Inc. 2012. p. 253-262. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-806-1_13

Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer

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