Detection of specific strains of viable bacterial pathogens by using RNA bead assays and flow cytometry with 2100 bioanalyzer

Philip Butterworth, Henrique T. M. C. M. Baltar, Martin Kratzmeier, Ewa M. Goldys*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Bead assays are an emerging microbial detection technology with the capability for rapid detection of extremely low levels of viable pathogens. Such technologies are of high value in clinical settings and in the food industry. Here, we perform a bead assay for extracted 16S rRNA from Escherichia coli (strain K12) with the flow cytometry readout on a 2100 Bioanalyzer, a highly accurate, small-scale flow cytometer system.

Original languageEnglish
Title of host publicationSpectroscopic Methods of Analysis
Subtitle of host publicationMethods and Protocols
EditorsWlodek M. Bujalowski
Place of PublicationNew York
PublisherHumana Press Inc.
Pages253-262
Number of pages10
ISBN (Electronic)9781617798061
ISBN (Print)9781617798054
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume875
ISSN (Print)1064-3745

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