Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin

Thomas S. Lawson*, Russell E. Connally, Jonathan R. Iredell, Subramanyam Vemulpad, James A. Piper

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

To detect with whole-cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lyozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme-only permeabilization. We compared lysozyme permeabilizationto S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1hr protocol is a useful alternative to conventional FISH.

Original languageEnglish
Pages (from-to)142-147
Number of pages6
JournalJournal of Clinical Laboratory Analysis
Volume25
Issue number2
DOIs
Publication statusPublished - 2011

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