TY - JOUR
T1 - Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin
AU - Lawson, Thomas S.
AU - Connally, Russell E.
AU - Iredell, Jonathan R.
AU - Vemulpad, Subramanyam
AU - Piper, James A.
PY - 2011
Y1 - 2011
N2 - To detect with whole-cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lyozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme-only permeabilization. We compared lysozyme permeabilizationto S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1hr protocol is a useful alternative to conventional FISH.
AB - To detect with whole-cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lyozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme-only permeabilization. We compared lysozyme permeabilizationto S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1hr protocol is a useful alternative to conventional FISH.
UR - http://www.scopus.com/inward/record.url?scp=79952668948&partnerID=8YFLogxK
U2 - 10.1002/jcla.20448
DO - 10.1002/jcla.20448
M3 - Article
C2 - 21438009
AN - SCOPUS:79952668948
SN - 0887-8013
VL - 25
SP - 142
EP - 147
JO - Journal of Clinical Laboratory Analysis
JF - Journal of Clinical Laboratory Analysis
IS - 2
ER -