Abstract
A microfluidic Lab-on-chip (LOC) platform for in vitro detecting Urinary Tract Infections (UTI) for clinical diagnostic applications has been built. Based on one commercial adenosine 5'-triphosphate (ATP) assay kit, one chip designed before was applied to detect UTI with the help of photomultiplier tube (PMT) and quantitative determination was made by measuring the photons of light emitted in the bioluminescent reaction of ATP with the enzyme luciferase. The chip had been tested and materials had been well prepared before testing the PMT detecting system. The data from PMT were visualized by the Labview™, appearing good linearity between voltage values and the concentration of the ATP ranging from 2×10-12 M to 2×10-8 M. Fresh urine sample with different amounts of Escherichia coli had been measured by the system, appearing good linearity trend between the voltage values and number of the E.coli. This study successfully expressed the concept of measuring ATP directly in the urine to quickly and accurately detect UTI on a microfluidic chip.
| Original language | English |
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| Title of host publication | EMBC 2014 |
| Subtitle of host publication | 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society |
| Place of Publication | Piscataway, NJ |
| Publisher | Institute of Electrical and Electronics Engineers (IEEE) |
| Pages | 3114-3117 |
| Number of pages | 4 |
| ISBN (Electronic) | 9781424479290 |
| DOIs | |
| Publication status | Published - 2 Nov 2014 |
| Externally published | Yes |
| Event | 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 - Chicago, United States Duration: 26 Aug 2014 → 30 Aug 2014 |
Other
| Other | 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2014 |
|---|---|
| Country/Territory | United States |
| City | Chicago |
| Period | 26/08/14 → 30/08/14 |