Determination of Effective Ingredients in Compound Sulfamethoxazole Tablets by Capillary Electrophoresis with Amperometric Detection

Chu Qing-Cui, Tian Xiu-Hui, Jiang Lian-Mei, Zhou Wen-Jun, Ye Jian-Nong*

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A high-performance capillary electrophoresis with amperometric detection (CE-AD) method has been used for the determination of three antibacterial sulfa components, sulfamethoxazole (SMZ), sulfadiazine (SD) and trimethoprim (TMP). The effects of several factors on CE-AD were investigated. Under the optimum conditions, the three components could be separated well within 14 min in a 70-cm length capillary at the separation voltage of 18 kV, injection time of 6 s in a 13-mM borax-18 mM potassium dihydrogen phosphate running buffer (pH 5.8). The above three analytes have good linear relationship between peak current and concentration of analytes in the range of 5 × 10-4-5 × 10 -2 g l-1, 5 × 10-4-1 × 10-1 g l-1, and 5 × 10-4-5 × 10-2 g l-1, respectively, and the detection limits (S/N = 3) ranged from 8.0 × 10-5 to 5.1 × 10-5 g l-1 for all analytes. This proposed method has been successfully applied for the determination of the main effective components in Compound Sulfamethoxazole Tablets.

Original languageEnglish
Pages (from-to)292-296
Number of pages5
JournalChinese Journal of Analytical Chemistry
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 2008
Externally publishedYes

Keywords

  • Amperometric detection
  • Capillary electrophoresis
  • Compound Sulfamethoxazole Tablets
  • Sulfadiazine
  • Sulfamethoxazole
  • Trimethoprim

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