TY - JOUR
T1 - Determination of Effective Ingredients in Compound Sulfamethoxazole Tablets by Capillary Electrophoresis with Amperometric Detection
AU - Qing-Cui, Chu
AU - Xiu-Hui, Tian
AU - Lian-Mei, Jiang
AU - Wen-Jun, Zhou
AU - Jian-Nong, Ye
PY - 2008/3
Y1 - 2008/3
N2 - A high-performance capillary electrophoresis with amperometric detection (CE-AD) method has been used for the determination of three antibacterial sulfa components, sulfamethoxazole (SMZ), sulfadiazine (SD) and trimethoprim (TMP). The effects of several factors on CE-AD were investigated. Under the optimum conditions, the three components could be separated well within 14 min in a 70-cm length capillary at the separation voltage of 18 kV, injection time of 6 s in a 13-mM borax-18 mM potassium dihydrogen phosphate running buffer (pH 5.8). The above three analytes have good linear relationship between peak current and concentration of analytes in the range of 5 × 10-4-5 × 10 -2 g l-1, 5 × 10-4-1 × 10-1 g l-1, and 5 × 10-4-5 × 10-2 g l-1, respectively, and the detection limits (S/N = 3) ranged from 8.0 × 10-5 to 5.1 × 10-5 g l-1 for all analytes. This proposed method has been successfully applied for the determination of the main effective components in Compound Sulfamethoxazole Tablets.
AB - A high-performance capillary electrophoresis with amperometric detection (CE-AD) method has been used for the determination of three antibacterial sulfa components, sulfamethoxazole (SMZ), sulfadiazine (SD) and trimethoprim (TMP). The effects of several factors on CE-AD were investigated. Under the optimum conditions, the three components could be separated well within 14 min in a 70-cm length capillary at the separation voltage of 18 kV, injection time of 6 s in a 13-mM borax-18 mM potassium dihydrogen phosphate running buffer (pH 5.8). The above three analytes have good linear relationship between peak current and concentration of analytes in the range of 5 × 10-4-5 × 10 -2 g l-1, 5 × 10-4-1 × 10-1 g l-1, and 5 × 10-4-5 × 10-2 g l-1, respectively, and the detection limits (S/N = 3) ranged from 8.0 × 10-5 to 5.1 × 10-5 g l-1 for all analytes. This proposed method has been successfully applied for the determination of the main effective components in Compound Sulfamethoxazole Tablets.
KW - Amperometric detection
KW - Capillary electrophoresis
KW - Compound Sulfamethoxazole Tablets
KW - Sulfadiazine
KW - Sulfamethoxazole
KW - Trimethoprim
UR - http://www.scopus.com/inward/record.url?scp=42049107330&partnerID=8YFLogxK
U2 - 10.1016/S1872-2040(08)60023-7
DO - 10.1016/S1872-2040(08)60023-7
M3 - Article
AN - SCOPUS:42049107330
SN - 1872-2040
VL - 36
SP - 292
EP - 296
JO - Chinese Journal of Analytical Chemistry
JF - Chinese Journal of Analytical Chemistry
IS - 3
ER -