Determination of interactions between tegument proteins of herpes simplex virus type 1

Valerio Vittone, Eve Diefenbach, Damian Triffett, Mark W Douglas, Anthony L. Cunningham, Russell J Diefenbach

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

The aim of this study was to elucidate protein-protein interactions between tegument proteins of herpes simplex virus type 1 (HSV-1). To do so, we have cloned and expressed in the LexA yeast (Saccharomyces cerevisiae) two-hybrid system, 13 of the 21 currently known tegument proteins of HSV-1. These included the tegument proteins essential for replication in cell lines, UL17, UL36, UL37, UL48, and UL49, and the nonessential tegument proteins US11, UL11, UL14, UL16, UL21, UL41, UL46, and UL47. A total of 104 combinations were screened in the yeast two-hybrid assay, with 9 interactions identified. These included: UL11-UL16, UL36-UL37, UL36-UL48, UL46-UL48, UL47-UL48, and UL48-UL49. The remaining interactions consisted of self-associations that were observed for US11, UL37, and UL49. The interactions UL36-UL37, UL36-UL48, UL37-UL37, UL46-UL48, and UL47-UL48 have not been previously reported for HSV-1. The interaction of UL46-UL48 was verified using an in vitro pull-down assay. The interactions of UL36-UL37 and UL37-UL37 were verified with a coimmunoprecipitation assay. Knowledge of HSV-1 tegument protein-protein interactions will provide insights into the pathways of tegument assembly, and the identified interactions are potential targets for new antiviral drugs.

Original languageEnglish
Pages (from-to)9566-9571
Number of pages6
JournalJournal of Virology
Volume79
Issue number15
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Keywords

  • HeLa Cells
  • Herpesvirus 1, Human
  • Humans
  • Protein Binding
  • Two-Hybrid System Techniques
  • Viral Envelope Proteins
  • Virus Assembly
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

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