Determination of isotope ratios in human tissues enriched with zinc stable isotope tracers using inductively coupled plasma-mass spectrometry (ICP-MS)

James K. Friel*, Henry P. Longerich, Simon E. Jackson

*Corresponding author for this work

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Enriched fecal and urine samples were prepared using ion-exchange column chromatography for analysis by Inductively Coupled and Fast Atom Bombardment Mass spectrometry (ICP-MS, FAB-MS) to compare precision between methods. Unenriched samples of human milk, feces, and whole blood were prepared similarly to monitor instrumental precision and analytical error. A least squares fit of the ICP-MS results vs the FAB-MS for70Zn/64Zn gave a slope of 0.98, with a relative standard deviation (RSD) of only 0.7%. The results for68Zn/64Zn gave a slope of 0.82, with a RSD of 14%. For unenriched tissues, all potential interferences were removed by the preparation procedure with no significant differences between preparation for isotope ratios of70Zn/64Zn,68Zn/64Zn,67Zn/64Zn, and66Zn/64Zn. Poisson counting statistics are a major contribution to the total analytical error indicating the usefulness of this procedure for enrichment studies.

    Original languageEnglish
    Pages (from-to)123-136
    Number of pages14
    JournalBiological Trace Element Research
    Volume37
    Issue number2-3
    DOIs
    Publication statusPublished - May 1993

    Keywords

    • human studies
    • ICP-MS
    • stable isotopes
    • Zinc

    Fingerprint Dive into the research topics of 'Determination of isotope ratios in human tissues enriched with zinc stable isotope tracers using inductively coupled plasma-mass spectrometry (ICP-MS)'. Together they form a unique fingerprint.

  • Cite this