Development of 21 microsatellite markers for the threatened Yarra pygmy perch (Nannoperca obscura) through 454 shot-gun pyrosequencing

Daniel C. Carvalho, Clara J. Rodríguez-Zárate, Michael P. Hammer, Luciano B. Beheregaray

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Using a next generation sequencing approach, a set of 21 new microsatellites loci was developed from the threatened freshwater fish Yarra pygmy perch (YPP) Nannoperca obscura (Percichthyidae). All markers were successfully genotyped using 28 YPP kept in captivity. These animals represent an entire population rescued from the lower Murray-Darling Basin in Australia before its habitat dried out. As expected due to the critical conservation status of the population, we observed low genetic variation across most loci (mean number of alleles per locus = 2.76; mean heterozygosity = 0.28). No deviations from Hardy-Weinberg equilibrium or linkage disequilibrium were detected and only one locus (Nob30) showed evidence for null alleles. These molecular markers represent important resources for the ongoing YPP captive breeding program, and for upcoming restoration and landscape genetic studies of this species.

Original languageEnglish
Pages (from-to)601-604
Number of pages4
JournalConservation Genetics Resources
Volume3
Issue number4
DOIs
Publication statusPublished - Oct 2011

Keywords

  • Freshwater fish
  • Microsatellite development
  • Nannoperca obscura
  • Next Generation DNA sequencing

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