Development of a functional ruthenium(II) complex for probing hypochlorous acid in living cells

Zhiqiang Ye; Run Zhang; Bo Song; Zhichao Dai; Dayong Jin; Ewa M. Goldys; Jingli Yuan

doi:10.1039/c4dt00179f

Development of a functional ruthenium(II) complex for probing hypochlorous acid in living cells

Zhiqiang Ye, Run Zhang, Bo Song, Zhichao Dai, Dayong Jin, Ewa M. Goldys, Jingli Yuan^*

^*Corresponding author for this work

MQ Photonics Research Centre

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

A functional ruthenium(ii) complex, [Ru(bpy)₂(AN-bpy)](PF ₆)₂ (bpy: 2,2′-bipyridine, AN-bpy: 4-methyl-4′-(4-amino-3-nitro-phenoxy-methylene)-2,2′-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(ii) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, [Ru(bpy) ₂(HM-bpy)](PF₆)₂ (HM-bpy: 4-hydroxymethyl- 4′-methyl-2,2′-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(ii) complex probe, [Ru(bpy) ₂(AN-bpy)](PF₆)₂ was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed [Ru(bpy)₂(AN-bpy)](PF₆)₂ is a useful luminescent probe for the monitoring of HOCl in biological systems.

Original language	English
Pages (from-to)	8414-8420
Number of pages	7
Journal	Dalton Transactions
Volume	43
Issue number	22
DOIs	https://doi.org/10.1039/c4dt00179f
Publication status	Published - 14 Jun 2014

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title = "Development of a functional ruthenium(II) complex for probing hypochlorous acid in living cells",

abstract = "A functional ruthenium(ii) complex, [Ru(bpy)2(AN-bpy)](PF 6)2 (bpy: 2,2′-bipyridine, AN-bpy: 4-methyl-4′-(4-amino-3-nitro-phenoxy-methylene)-2,2′-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(ii) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, [Ru(bpy) 2(HM-bpy)](PF6)2 (HM-bpy: 4-hydroxymethyl- 4′-methyl-2,2′-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(ii) complex probe, [Ru(bpy) 2(AN-bpy)](PF6)2 was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed [Ru(bpy)2(AN-bpy)](PF6)2 is a useful luminescent probe for the monitoring of HOCl in biological systems.",

author = "Zhiqiang Ye and Run Zhang and Bo Song and Zhichao Dai and Dayong Jin and Goldys, {Ewa M.} and Jingli Yuan",

year = "2014",

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AU - Zhang, Run

AU - Song, Bo

AU - Dai, Zhichao

AU - Jin, Dayong

AU - Goldys, Ewa M.

AU - Yuan, Jingli

PY - 2014/6/14

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N2 - A functional ruthenium(ii) complex, [Ru(bpy)2(AN-bpy)](PF 6)2 (bpy: 2,2′-bipyridine, AN-bpy: 4-methyl-4′-(4-amino-3-nitro-phenoxy-methylene)-2,2′-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(ii) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, [Ru(bpy) 2(HM-bpy)](PF6)2 (HM-bpy: 4-hydroxymethyl- 4′-methyl-2,2′-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(ii) complex probe, [Ru(bpy) 2(AN-bpy)](PF6)2 was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed [Ru(bpy)2(AN-bpy)](PF6)2 is a useful luminescent probe for the monitoring of HOCl in biological systems.

AB - A functional ruthenium(ii) complex, [Ru(bpy)2(AN-bpy)](PF 6)2 (bpy: 2,2′-bipyridine, AN-bpy: 4-methyl-4′-(4-amino-3-nitro-phenoxy-methylene)-2,2′-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(ii) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, [Ru(bpy) 2(HM-bpy)](PF6)2 (HM-bpy: 4-hydroxymethyl- 4′-methyl-2,2′-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(ii) complex probe, [Ru(bpy) 2(AN-bpy)](PF6)2 was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed [Ru(bpy)2(AN-bpy)](PF6)2 is a useful luminescent probe for the monitoring of HOCl in biological systems.

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Development of a functional ruthenium(II) complex for probing hypochlorous acid in living cells

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