Development of a functional ruthenium(II) complex for probing hypochlorous acid in living cells

Zhiqiang Ye, Run Zhang, Bo Song, Zhichao Dai, Dayong Jin, Ewa M. Goldys, Jingli Yuan*

*Corresponding author for this work

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A functional ruthenium(ii) complex, [Ru(bpy)2(AN-bpy)](PF 6)2 (bpy: 2,2′-bipyridine, AN-bpy: 4-methyl-4′-(4-amino-3-nitro-phenoxy-methylene)-2,2′-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(ii) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, [Ru(bpy) 2(HM-bpy)](PF6)2 (HM-bpy: 4-hydroxymethyl- 4′-methyl-2,2′-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(ii) complex probe, [Ru(bpy) 2(AN-bpy)](PF6)2 was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed [Ru(bpy)2(AN-bpy)](PF6)2 is a useful luminescent probe for the monitoring of HOCl in biological systems.

Original languageEnglish
Pages (from-to)8414-8420
Number of pages7
JournalDalton Transactions
Volume43
Issue number22
DOIs
Publication statusPublished - 14 Jun 2014

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