Development of a polysaccharide degrading strain of Saccharomyces cerevisiae

S. H. Petersen, W. H. Van Zyl, I. S. Pretorius*

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Saccharomyces cerevisiae lacks most of the depolymerising enzymes required for efficient hydrolysis and utilisation of polysaccharide-rich biomass. To allow extracellular degradation of polysaccharides, genes encoding amylopullulanase (LKA1), pectate lyase (PEL5), polygalacturonase (PEH1), endo-β-1,4-D-glucanase (END1), cellobiohydrolase (CBH1), exo-β-1,3-D-glucanase (EXG1), cellobiase (β-glucosidase; BGL1) and endo-β-D-xylanase (XYN4) were introduced jointly into a laboratory strain of S. cerevisiae. These transformants were able to grow on starch, pectate, cellobiose and to some extent on cellulose (solka-floc and lichenan). These results pave the way for the development of one-step bioconversion processing of plant biomass in the fuel, animal feed, baking and beverage industries.

Original languageEnglish
Pages (from-to)615-619
Number of pages5
JournalBiotechnology Techniques
Volume12
Issue number8
DOIs
Publication statusPublished - 1998
Externally publishedYes

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