Development of a polysaccharide degrading strain of Saccharomyces cerevisiae

S. H. Petersen; W. H. Van Zyl; I. S. Pretorius

doi:10.1023/A:1008829129516

Development of a polysaccharide degrading strain of Saccharomyces cerevisiae

S. H. Petersen, W. H. Van Zyl, I. S. Pretorius^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Saccharomyces cerevisiae lacks most of the depolymerising enzymes required for efficient hydrolysis and utilisation of polysaccharide-rich biomass. To allow extracellular degradation of polysaccharides, genes encoding amylopullulanase (LKA1), pectate lyase (PEL5), polygalacturonase (PEH1), endo-β-1,4-D-glucanase (END1), cellobiohydrolase (CBH1), exo-β-1,3-D-glucanase (EXG1), cellobiase (β-glucosidase; BGL1) and endo-β-D-xylanase (XYN4) were introduced jointly into a laboratory strain of S. cerevisiae. These transformants were able to grow on starch, pectate, cellobiose and to some extent on cellulose (solka-floc and lichenan). These results pave the way for the development of one-step bioconversion processing of plant biomass in the fuel, animal feed, baking and beverage industries.

Original language	English
Pages (from-to)	615-619
Number of pages	5
Journal	Biotechnology Techniques
Volume	12
Issue number	8
DOIs	https://doi.org/10.1023/A:1008829129516
Publication status	Published - 1998
Externally published	Yes

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title = "Development of a polysaccharide degrading strain of Saccharomyces cerevisiae",

abstract = "Saccharomyces cerevisiae lacks most of the depolymerising enzymes required for efficient hydrolysis and utilisation of polysaccharide-rich biomass. To allow extracellular degradation of polysaccharides, genes encoding amylopullulanase (LKA1), pectate lyase (PEL5), polygalacturonase (PEH1), endo-β-1,4-D-glucanase (END1), cellobiohydrolase (CBH1), exo-β-1,3-D-glucanase (EXG1), cellobiase (β-glucosidase; BGL1) and endo-β-D-xylanase (XYN4) were introduced jointly into a laboratory strain of S. cerevisiae. These transformants were able to grow on starch, pectate, cellobiose and to some extent on cellulose (solka-floc and lichenan). These results pave the way for the development of one-step bioconversion processing of plant biomass in the fuel, animal feed, baking and beverage industries.",

author = "Petersen, {S. H.} and {Van Zyl}, {W. H.} and Pretorius, {I. S.}",

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T1 - Development of a polysaccharide degrading strain of Saccharomyces cerevisiae

AU - Petersen, S. H.

AU - Van Zyl, W. H.

AU - Pretorius, I. S.

PY - 1998

Y1 - 1998

N2 - Saccharomyces cerevisiae lacks most of the depolymerising enzymes required for efficient hydrolysis and utilisation of polysaccharide-rich biomass. To allow extracellular degradation of polysaccharides, genes encoding amylopullulanase (LKA1), pectate lyase (PEL5), polygalacturonase (PEH1), endo-β-1,4-D-glucanase (END1), cellobiohydrolase (CBH1), exo-β-1,3-D-glucanase (EXG1), cellobiase (β-glucosidase; BGL1) and endo-β-D-xylanase (XYN4) were introduced jointly into a laboratory strain of S. cerevisiae. These transformants were able to grow on starch, pectate, cellobiose and to some extent on cellulose (solka-floc and lichenan). These results pave the way for the development of one-step bioconversion processing of plant biomass in the fuel, animal feed, baking and beverage industries.

AB - Saccharomyces cerevisiae lacks most of the depolymerising enzymes required for efficient hydrolysis and utilisation of polysaccharide-rich biomass. To allow extracellular degradation of polysaccharides, genes encoding amylopullulanase (LKA1), pectate lyase (PEL5), polygalacturonase (PEH1), endo-β-1,4-D-glucanase (END1), cellobiohydrolase (CBH1), exo-β-1,3-D-glucanase (EXG1), cellobiase (β-glucosidase; BGL1) and endo-β-D-xylanase (XYN4) were introduced jointly into a laboratory strain of S. cerevisiae. These transformants were able to grow on starch, pectate, cellobiose and to some extent on cellulose (solka-floc and lichenan). These results pave the way for the development of one-step bioconversion processing of plant biomass in the fuel, animal feed, baking and beverage industries.

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DO - 10.1023/A:1008829129516

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JO - Biotechnology Techniques

JF - Biotechnology Techniques

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Development of a polysaccharide degrading strain of Saccharomyces cerevisiae

Abstract

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