Development of a sensing system to detect C-telopeptide of type-I collagen

Nasrin Afsarimanesh, Asif I. Zia, S. C. Mukhopadhyay, Marlena Kruger, P. L. Yu, Jurgen Kosel

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

Abstract

This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.

Original languageEnglish
Title of host publicationICST 2015
Subtitle of host publicationproceedings of the 9th International Conference on Sensing Technology
Place of PublicationPiscataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages586-590
Number of pages5
ISBN (Electronic)9781479963140
DOIs
Publication statusPublished - 2015
Externally publishedYes
Event9th International Conference on Sensing Technology, ICST 2015 - Auckland, New Zealand
Duration: 8 Dec 201511 Dec 2015

Other

Other9th International Conference on Sensing Technology, ICST 2015
CountryNew Zealand
CityAuckland
Period8/12/1511/12/15

Keywords

  • Electrochemical Impedance Spectroscopy
  • ELISA
  • interdigital sensor
  • osteoporosis

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