Development of a sensing system to detect C-telopeptide of type-I collagen

Nasrin Afsarimanesh, Asif I. Zia, S. C. Mukhopadhyay, Marlena Kruger, P. L. Yu, Jurgen Kosel

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionResearchpeer-review

Abstract

This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.

LanguageEnglish
Title of host publicationICST 2015
Subtitle of host publicationproceedings of the 9th International Conference on Sensing Technology
Place of PublicationPiscataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages586-590
Number of pages5
ISBN (Electronic)9781479963140
DOIs
Publication statusPublished - 2015
Externally publishedYes
Event9th International Conference on Sensing Technology, ICST 2015 - Auckland, New Zealand
Duration: 8 Dec 201511 Dec 2015

Other

Other9th International Conference on Sensing Technology, ICST 2015
CountryNew Zealand
CityAuckland
Period8/12/1511/12/15

Fingerprint

Collagen
Electrochemical impedance spectroscopy
Sensors
Capacitive sensors
Semiconductor devices
Antibodies
MEMS
Labels
Blood
Plasmas
Fabrication
Thin films
Experiments

Keywords

  • Electrochemical Impedance Spectroscopy
  • ELISA
  • interdigital sensor
  • osteoporosis

Cite this

Afsarimanesh, N., Zia, A. I., Mukhopadhyay, S. C., Kruger, M., Yu, P. L., & Kosel, J. (2015). Development of a sensing system to detect C-telopeptide of type-I collagen. In ICST 2015: proceedings of the 9th International Conference on Sensing Technology (pp. 586-590). Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/ICSensT.2015.7438466
Afsarimanesh, Nasrin ; Zia, Asif I. ; Mukhopadhyay, S. C. ; Kruger, Marlena ; Yu, P. L. ; Kosel, Jurgen. / Development of a sensing system to detect C-telopeptide of type-I collagen. ICST 2015: proceedings of the 9th International Conference on Sensing Technology. Piscataway, NJ : Institute of Electrical and Electronics Engineers (IEEE), 2015. pp. 586-590
@inproceedings{a12a80c0cd014b1499c49df0cf50cc66,
title = "Development of a sensing system to detect C-telopeptide of type-I collagen",
abstract = "This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps{\circledR} ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.",
keywords = "Electrochemical Impedance Spectroscopy, ELISA, interdigital sensor, osteoporosis",
author = "Nasrin Afsarimanesh and Zia, {Asif I.} and Mukhopadhyay, {S. C.} and Marlena Kruger and Yu, {P. L.} and Jurgen Kosel",
year = "2015",
doi = "10.1109/ICSensT.2015.7438466",
language = "English",
pages = "586--590",
booktitle = "ICST 2015",
publisher = "Institute of Electrical and Electronics Engineers (IEEE)",
address = "United States",

}

Afsarimanesh, N, Zia, AI, Mukhopadhyay, SC, Kruger, M, Yu, PL & Kosel, J 2015, Development of a sensing system to detect C-telopeptide of type-I collagen. in ICST 2015: proceedings of the 9th International Conference on Sensing Technology. Institute of Electrical and Electronics Engineers (IEEE), Piscataway, NJ, pp. 586-590, 9th International Conference on Sensing Technology, ICST 2015, Auckland, New Zealand, 8/12/15. https://doi.org/10.1109/ICSensT.2015.7438466

Development of a sensing system to detect C-telopeptide of type-I collagen. / Afsarimanesh, Nasrin; Zia, Asif I.; Mukhopadhyay, S. C.; Kruger, Marlena; Yu, P. L.; Kosel, Jurgen.

ICST 2015: proceedings of the 9th International Conference on Sensing Technology. Piscataway, NJ : Institute of Electrical and Electronics Engineers (IEEE), 2015. p. 586-590.

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionResearchpeer-review

TY - GEN

T1 - Development of a sensing system to detect C-telopeptide of type-I collagen

AU - Afsarimanesh, Nasrin

AU - Zia, Asif I.

AU - Mukhopadhyay, S. C.

AU - Kruger, Marlena

AU - Yu, P. L.

AU - Kosel, Jurgen

PY - 2015

Y1 - 2015

N2 - This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.

AB - This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.

KW - Electrochemical Impedance Spectroscopy

KW - ELISA

KW - interdigital sensor

KW - osteoporosis

UR - http://www.scopus.com/inward/record.url?scp=84964814961&partnerID=8YFLogxK

U2 - 10.1109/ICSensT.2015.7438466

DO - 10.1109/ICSensT.2015.7438466

M3 - Conference proceeding contribution

SP - 586

EP - 590

BT - ICST 2015

PB - Institute of Electrical and Electronics Engineers (IEEE)

CY - Piscataway, NJ

ER -

Afsarimanesh N, Zia AI, Mukhopadhyay SC, Kruger M, Yu PL, Kosel J. Development of a sensing system to detect C-telopeptide of type-I collagen. In ICST 2015: proceedings of the 9th International Conference on Sensing Technology. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). 2015. p. 586-590 https://doi.org/10.1109/ICSensT.2015.7438466