Abstract
This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.
Original language | English |
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Title of host publication | ICST 2015 |
Subtitle of host publication | proceedings of the 9th International Conference on Sensing Technology |
Place of Publication | Piscataway, NJ |
Publisher | Institute of Electrical and Electronics Engineers (IEEE) |
Pages | 586-590 |
Number of pages | 5 |
ISBN (Electronic) | 9781479963140 |
DOIs | |
Publication status | Published - 2015 |
Externally published | Yes |
Event | 9th International Conference on Sensing Technology, ICST 2015 - Auckland, New Zealand Duration: 8 Dec 2015 → 11 Dec 2015 |
Other
Other | 9th International Conference on Sensing Technology, ICST 2015 |
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Country/Territory | New Zealand |
City | Auckland |
Period | 8/12/15 → 11/12/15 |
Keywords
- Electrochemical Impedance Spectroscopy
- ELISA
- interdigital sensor
- osteoporosis