Development of a sensing system to detect C-telopeptide of type-I collagen

Nasrin Afsarimanesh; Asif I. Zia; S. C. Mukhopadhyay; Marlena Kruger; P. L. Yu; Jurgen Kosel

doi:10.1109/ICSensT.2015.7438466

Development of a sensing system to detect C-telopeptide of type-I collagen

Nasrin Afsarimanesh, Asif I. Zia, S. C. Mukhopadhyay, Marlena Kruger, P. L. Yu, Jurgen Kosel

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.

Original language	English
Title of host publication	ICST 2015
Subtitle of host publication	proceedings of the 9th International Conference on Sensing Technology
Place of Publication	Piscataway, NJ
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	586-590
Number of pages	5
ISBN (Electronic)	9781479963140
DOIs	https://doi.org/10.1109/ICSensT.2015.7438466
Publication status	Published - 2015
Externally published	Yes
Event	9th International Conference on Sensing Technology, ICST 2015 - Auckland, New Zealand Duration: 8 Dec 2015 → 11 Dec 2015

Other

Other	9th International Conference on Sensing Technology, ICST 2015
Country/Territory	New Zealand
City	Auckland
Period	8/12/15 → 11/12/15

Keywords

Electrochemical Impedance Spectroscopy
ELISA
interdigital sensor
osteoporosis

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10.1109/ICSensT.2015.7438466

Cite this

Afsarimanesh, N., Zia, A. I., Mukhopadhyay, S. C., Kruger, M., Yu, P. L., & Kosel, J. (2015). Development of a sensing system to detect C-telopeptide of type-I collagen. In ICST 2015: proceedings of the 9th International Conference on Sensing Technology (pp. 586-590). Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/ICSensT.2015.7438466

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title = "Development of a sensing system to detect C-telopeptide of type-I collagen",

abstract = "This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps{\textregistered} ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.",

keywords = "Electrochemical Impedance Spectroscopy, ELISA, interdigital sensor, osteoporosis",

author = "Nasrin Afsarimanesh and Zia, \{Asif I.\} and Mukhopadhyay, \{S. C.\} and Marlena Kruger and Yu, \{P. L.\} and Jurgen Kosel",

year = "2015",

doi = "10.1109/ICSensT.2015.7438466",

language = "English",

pages = "586--590",

booktitle = "ICST 2015",

publisher = "Institute of Electrical and Electronics Engineers (IEEE)",

address = "United States",

note = "9th International Conference on Sensing Technology, ICST 2015 ; Conference date: 08-12-2015 Through 11-12-2015",

}

Afsarimanesh, N, Zia, AI, Mukhopadhyay, SC, Kruger, M, Yu, PL & Kosel, J 2015, Development of a sensing system to detect C-telopeptide of type-I collagen. in ICST 2015: proceedings of the 9th International Conference on Sensing Technology. Institute of Electrical and Electronics Engineers (IEEE), Piscataway, NJ, pp. 586-590, 9th International Conference on Sensing Technology, ICST 2015, Auckland, New Zealand, 8/12/15. https://doi.org/10.1109/ICSensT.2015.7438466

Development of a sensing system to detect C-telopeptide of type-I collagen. / Afsarimanesh, Nasrin; Zia, Asif I.; Mukhopadhyay, S. C. et al.
ICST 2015: proceedings of the 9th International Conference on Sensing Technology. Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE), 2015. p. 586-590.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Development of a sensing system to detect C-telopeptide of type-I collagen

AU - Afsarimanesh, Nasrin

AU - Zia, Asif I.

AU - Mukhopadhyay, S. C.

AU - Kruger, Marlena

AU - Yu, P. L.

AU - Kosel, Jurgen

PY - 2015

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N2 - This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.

AB - This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.

KW - Electrochemical Impedance Spectroscopy

KW - ELISA

KW - interdigital sensor

KW - osteoporosis

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DO - 10.1109/ICSensT.2015.7438466

M3 - Conference proceeding contribution

SP - 586

EP - 590

BT - ICST 2015

PB - Institute of Electrical and Electronics Engineers (IEEE)

CY - Piscataway, NJ

T2 - 9th International Conference on Sensing Technology, ICST 2015

Y2 - 8 December 2015 through 11 December 2015

ER -

Development of a sensing system to detect C-telopeptide of type-I collagen

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