Development of a sensitive HPLC method to measure in vitro permeability of E- and Z-isomeric forms of thiosemicarbazones in Caco-2 monolayers

Zufan Debebe; Sergei Nekhai; Meseret Ashenafi; David B. Lovejoy; Danuta S. Kalinowski; Victor R. Gordeuk; W. Malcolm Byrnes; Des R. Richardson; Pradeep K. Karla

doi:10.1016/j.jchromb.2012.08.011

Development of a sensitive HPLC method to measure in vitro permeability of E- and Z-isomeric forms of thiosemicarbazones in Caco-2 monolayers

Zufan Debebe, Sergei Nekhai, Meseret Ashenafi, David B. Lovejoy, Danuta S. Kalinowski, Victor R. Gordeuk, W. Malcolm Byrnes, Des R. Richardson, Pradeep K. Karla^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

In the current study, we developed a HPLC method to quantitatively measure the permeability of the BpT-based chelators, 2-benzoylpyridine 4-ethyl-3-thiosemicarbazone (Bp4eT) and 2-benzoylpyridine 4-allyl-3-thiosemicarbazone (Bp4aT), across human colorectal adenocarcinoma (Caco-2) monolayers as a model of gut absorption. In aqueous solution, Bp4eT and Bp4aT formed inter-convertible Z and E isomers that were resolved by HPLC. Peak area was linear with respect to chelator concentration. Acceptable within-day and between-day precision (

Original language	English
Pages (from-to)	25-32
Number of pages	8
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	906
DOIs	https://doi.org/10.1016/j.jchromb.2012.08.011
Publication status	Published - 1 Oct 2012
Externally published	Yes

Keywords

HIV-1 iron chelator
HPLC method development
Inter-convertible Z and E isomers
Caco2 drug permeability
INHIBIT HIV-1 TRANSCRIPTION
IRON CHELATORS
GENE-EXPRESSION
CELLS
TRANSPORT
DESFERRIOXAMINE
MECHANISMS
SERIES
IDENTIFICATION
DEFERIPRONE

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10.1016/j.jchromb.2012.08.011

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Debebe, Z., Nekhai, S., Ashenafi, M., Lovejoy, D. B., Kalinowski, D. S., Gordeuk, V. R., Byrnes, W. M., Richardson, D. R., & Karla, P. K. (2012). Development of a sensitive HPLC method to measure in vitro permeability of E- and Z-isomeric forms of thiosemicarbazones in Caco-2 monolayers. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 906, 25-32. https://doi.org/10.1016/j.jchromb.2012.08.011

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title = "Development of a sensitive HPLC method to measure in vitro permeability of E- and Z-isomeric forms of thiosemicarbazones in Caco-2 monolayers",

abstract = "In the current study, we developed a HPLC method to quantitatively measure the permeability of the BpT-based chelators, 2-benzoylpyridine 4-ethyl-3-thiosemicarbazone (Bp4eT) and 2-benzoylpyridine 4-allyl-3-thiosemicarbazone (Bp4aT), across human colorectal adenocarcinoma (Caco-2) monolayers as a model of gut absorption. In aqueous solution, Bp4eT and Bp4aT formed inter-convertible Z and E isomers that were resolved by HPLC. Peak area was linear with respect to chelator concentration. Acceptable within-day and between-day precision (",

keywords = "HIV-1 iron chelator, HPLC method development, Inter-convertible Z and E isomers, Caco2 drug permeability, INHIBIT HIV-1 TRANSCRIPTION, IRON CHELATORS, GENE-EXPRESSION, CELLS, TRANSPORT, DESFERRIOXAMINE, MECHANISMS, SERIES, IDENTIFICATION, DEFERIPRONE",

author = "Zufan Debebe and Sergei Nekhai and Meseret Ashenafi and Lovejoy, {David B.} and Kalinowski, {Danuta S.} and Gordeuk, {Victor R.} and Byrnes, {W. Malcolm} and Richardson, {Des R.} and Karla, {Pradeep K.}",

year = "2012",

month = oct,

day = "1",

doi = "10.1016/j.jchromb.2012.08.011",

language = "English",

volume = "906",

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journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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Debebe, Z, Nekhai, S, Ashenafi, M, Lovejoy, DB, Kalinowski, DS, Gordeuk, VR, Byrnes, WM, Richardson, DR & Karla, PK 2012, 'Development of a sensitive HPLC method to measure in vitro permeability of E- and Z-isomeric forms of thiosemicarbazones in Caco-2 monolayers', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 906, pp. 25-32. https://doi.org/10.1016/j.jchromb.2012.08.011

Development of a sensitive HPLC method to measure in vitro permeability of E- and Z-isomeric forms of thiosemicarbazones in Caco-2 monolayers. / Debebe, Zufan; Nekhai, Sergei; Ashenafi, Meseret et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 906, 01.10.2012, p. 25-32.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development of a sensitive HPLC method to measure in vitro permeability of E- and Z-isomeric forms of thiosemicarbazones in Caco-2 monolayers

AU - Debebe, Zufan

AU - Nekhai, Sergei

AU - Ashenafi, Meseret

AU - Lovejoy, David B.

AU - Kalinowski, Danuta S.

AU - Gordeuk, Victor R.

AU - Byrnes, W. Malcolm

AU - Richardson, Des R.

AU - Karla, Pradeep K.

PY - 2012/10/1

Y1 - 2012/10/1

N2 - In the current study, we developed a HPLC method to quantitatively measure the permeability of the BpT-based chelators, 2-benzoylpyridine 4-ethyl-3-thiosemicarbazone (Bp4eT) and 2-benzoylpyridine 4-allyl-3-thiosemicarbazone (Bp4aT), across human colorectal adenocarcinoma (Caco-2) monolayers as a model of gut absorption. In aqueous solution, Bp4eT and Bp4aT formed inter-convertible Z and E isomers that were resolved by HPLC. Peak area was linear with respect to chelator concentration. Acceptable within-day and between-day precision (

AB - In the current study, we developed a HPLC method to quantitatively measure the permeability of the BpT-based chelators, 2-benzoylpyridine 4-ethyl-3-thiosemicarbazone (Bp4eT) and 2-benzoylpyridine 4-allyl-3-thiosemicarbazone (Bp4aT), across human colorectal adenocarcinoma (Caco-2) monolayers as a model of gut absorption. In aqueous solution, Bp4eT and Bp4aT formed inter-convertible Z and E isomers that were resolved by HPLC. Peak area was linear with respect to chelator concentration. Acceptable within-day and between-day precision (

KW - HIV-1 iron chelator

KW - HPLC method development

KW - Inter-convertible Z and E isomers

KW - Caco2 drug permeability

KW - INHIBIT HIV-1 TRANSCRIPTION

KW - IRON CHELATORS

KW - GENE-EXPRESSION

KW - CELLS

KW - TRANSPORT

KW - DESFERRIOXAMINE

KW - MECHANISMS

KW - SERIES

KW - IDENTIFICATION

KW - DEFERIPRONE

U2 - 10.1016/j.jchromb.2012.08.011

DO - 10.1016/j.jchromb.2012.08.011

M3 - Article

C2 - 22954966

SN - 1570-0232

VL - 906

SP - 25

EP - 32

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

ER -

Development of a sensitive HPLC method to measure in vitro permeability of E- and Z-isomeric forms of thiosemicarbazones in Caco-2 monolayers

Abstract

Keywords

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