Abstract
We describe a simple, rapid method for protein complex purification in planta. Using a biotin peptide as an affinity tag with TATA-box binding protein (TBP), 86 unique proteins present in the purified complex were identified by tandem mass spectrometry. We identified proteins known to be associated with TBP, and many other proteins involved in pre-mRNA processing and chromatin remodeling. The identification of these novel protein-protein associations will upon further investigations provide new insights into the mechanisms of mRNA transcription and pre-mRNA processing.
Original language | English |
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Pages (from-to) | 514-522 |
Number of pages | 9 |
Journal | Journal of Proteome Research |
Volume | 2 |
Issue number | 5 |
DOIs | |
Publication status | Published - Sept 2003 |
Externally published | Yes |
Keywords
- HPLC-MS/MS
- Plant
- Protein complex
- Protein-protein interactions
- Tandem mass spectrometry
- TATA-box binding protein