Development of a System for the Study of Protein-Protein Interactions in Planta

Characterization of a TATA-Box Binding Protein Complex in Oryza sativa

Jingping Zhong, Paul A. Haynes, Shiping Zhang, Xinping Yang, Nancy L. Andon, Donna Eckert, John R. Yates, Xun Wang, Paul Budworth*

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We describe a simple, rapid method for protein complex purification in planta. Using a biotin peptide as an affinity tag with TATA-box binding protein (TBP), 86 unique proteins present in the purified complex were identified by tandem mass spectrometry. We identified proteins known to be associated with TBP, and many other proteins involved in pre-mRNA processing and chromatin remodeling. The identification of these novel protein-protein associations will upon further investigations provide new insights into the mechanisms of mRNA transcription and pre-mRNA processing.

Original languageEnglish
Pages (from-to)514-522
Number of pages9
JournalJournal of Proteome Research
Volume2
Issue number5
DOIs
Publication statusPublished - Sep 2003
Externally publishedYes

Keywords

  • HPLC-MS/MS
  • Plant
  • Protein complex
  • Protein-protein interactions
  • Tandem mass spectrometry
  • TATA-box binding protein

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