Development of a two-color fluorescence in situ hybridization technique for species-level identification of human-infectious Cryptosporidium spp.

A. Alagappan; Peter L. Bergquist; B. C. Ferrari

doi:10.1128/AEM.00643-09

Development of a two-color fluorescence in situ hybridization technique for species-level identification of human-infectious Cryptosporidium spp.

A. Alagappan, Peter L. Bergquist, B. C. Ferrari^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.

Original language	English
Pages (from-to)	5996-5998
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	18
DOIs	https://doi.org/10.1128/AEM.00643-09
Publication status	Published - 15 Sept 2009

Keywords

MOLECULAR CHARACTERIZATION
HIV
TRANSMISSION
GENOTYPES
ANIMALS
OOCYSTS
LIMA
PERU

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10.1128/AEM.00643-09

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title = "Development of a two-color fluorescence in situ hybridization technique for species-level identification of human-infectious Cryptosporidium spp.",

abstract = "A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.",

keywords = "MOLECULAR CHARACTERIZATION, HIV, TRANSMISSION, GENOTYPES, ANIMALS, OOCYSTS, LIMA, PERU",

author = "A. Alagappan and Bergquist, \{Peter L.\} and Ferrari, \{B. C.\}",

year = "2009",

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AU - Alagappan, A.

AU - Bergquist, Peter L.

AU - Ferrari, B. C.

PY - 2009/9/15

Y1 - 2009/9/15

N2 - A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.

AB - A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.

KW - MOLECULAR CHARACTERIZATION

KW - HIV

KW - TRANSMISSION

KW - GENOTYPES

KW - ANIMALS

KW - OOCYSTS

KW - LIMA

KW - PERU

UR - https://www.scopus.com/pages/publications/70149119692

U2 - 10.1128/AEM.00643-09

DO - 10.1128/AEM.00643-09

M3 - Article

C2 - 19633112

SN - 0099-2240

VL - 75

SP - 5996

EP - 5998

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 18

ER -

Development of a two-color fluorescence in situ hybridization technique for species-level identification of human-infectious Cryptosporidium spp.

Abstract

Keywords

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