TY - JOUR
T1 - Development of DNA-immobilised chromatographic stationary phases for optical resolution and DNA-affinity comparison of metal complexes
AU - Aldrich-Wright, Janice R.
AU - Greguric, Ivan
AU - Vagg, Robert S.
AU - Vickery, Kymberley
AU - Williams, Peter A.
PY - 1995/12/22
Y1 - 1995/12/22
N2 - Two chiral stationary phases which exploit DNA as a chiral discriminator have been developed. A covalently-bound DNA stationary phase for HPLC applications was used to optically resolve [Ru(dipyrido[6,7-d:2′,3′-f]-quinoxaline)3]2+ and [Ru(1,10-phenanthroline)3]2+ complex ions. This shows that the column retention times are influenced both by pH and by the size of the aromatic ligands. DNA-immobilised on cellulose paper proved effective for simultaneously comparing the relative retention of a number of metal complexes and RF data correlate well with the degree of aromatic area in the complexes available for intercalation into DNA.
AB - Two chiral stationary phases which exploit DNA as a chiral discriminator have been developed. A covalently-bound DNA stationary phase for HPLC applications was used to optically resolve [Ru(dipyrido[6,7-d:2′,3′-f]-quinoxaline)3]2+ and [Ru(1,10-phenanthroline)3]2+ complex ions. This shows that the column retention times are influenced both by pH and by the size of the aromatic ligands. DNA-immobilised on cellulose paper proved effective for simultaneously comparing the relative retention of a number of metal complexes and RF data correlate well with the degree of aromatic area in the complexes available for intercalation into DNA.
UR - http://www.scopus.com/inward/record.url?scp=0029584360&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(95)00703-2
DO - 10.1016/0021-9673(95)00703-2
M3 - Article
AN - SCOPUS:0029584360
SN - 0021-9673
VL - 718
SP - 436
EP - 443
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -