Development of DNA-immobilised chromatographic stationary phases for optical resolution and DNA-affinity comparison of metal complexes

Janice R. Aldrich-Wright*, Ivan Greguric, Robert S. Vagg, Kymberley Vickery, Peter A. Williams

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Two chiral stationary phases which exploit DNA as a chiral discriminator have been developed. A covalently-bound DNA stationary phase for HPLC applications was used to optically resolve [Ru(dipyrido[6,7-d:2′,3′-f]-quinoxaline)3]2+ and [Ru(1,10-phenanthroline)3]2+ complex ions. This shows that the column retention times are influenced both by pH and by the size of the aromatic ligands. DNA-immobilised on cellulose paper proved effective for simultaneously comparing the relative retention of a number of metal complexes and RF data correlate well with the degree of aromatic area in the complexes available for intercalation into DNA.

Original languageEnglish
Pages (from-to)436-443
Number of pages8
JournalJournal of Chromatography A
Volume718
Issue number2
DOIs
Publication statusPublished - 22 Dec 1995

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