Development of IoT-based impedometric biosensor for point-of-care monitoring of bone loss

Nasrin Afsarimanesh, Md. Eshrat E. Alahi, Subhas Chandra Mukhopadhyay, Marlena Kruger

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Early detection of disease is essential for an efficient treatment. Bone loss can be detected and monitored by regular measurement of serum or urine C-terminal telopeptide of type 1 collagen (CTx-1). Therefore, rapid, portable and low-cost point-of-care devices are highly desirable. In this paper, we have reported an IoT-based selective, sensitive, quick and inexpensive device for the quantification of CTx-1 levels in serum. A capacitive interdigital sensor was coated with artificial antibodies, prepared by molecular imprinting technology. Electrochemical impedance spectroscopy was used to evaluate the resistive and capacitive properties of the sample solutions. A microcontroller-based system was developed for the measurement of the level of CTx-1 in serum and for data transmission to an Internet of Things (IoT)-based cloud server. The data can be provided to the medical practitioner and a detailed investigation can start for early detection and treatment. The developed sensing system responded linearly in a range of 0.1 ppb to 2.5 ppb, which covers the normal reference range of CTx-1 in serum, with a limit of detection (LOD) of 0.09 ppb. The results demonstrated that the proposed portable biosensing system could provide a rapid, simple and selective approach for CTx-1 measurement in serum. Sheep serum samples were tested using the proposed system and the validation of the results was done using an enzyme-linked immunosorbent assay (ELISA) kit.

Original languageEnglish
Pages (from-to)211-220
Number of pages10
JournalIEEE Journal on Emerging and Selected Topics in Circuits and Systems
Volume8
Issue number2
DOIs
Publication statusPublished - Jun 2018

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