Development of mini-gel technology in two-dimensional electrophoresis for mass-screening of samples: application to tears

Melissa J. Glasson, Mark P. Molloy, Bradley J. Walsh, Mark D. P. Willcox, Carol A. Morris, Keith L. Williams

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Despite the extensive literature available on tear proteins and lipids, very little has been reported on the tear fluid as a whole and it's changes in contact lens wear or ocular diseased patients. Initially a human reflex tear two-dimensional map was created by Molloy et al. (Electrophoresis 1997, 18, 2811-2815), using format two-dimensional technique was evaluated, using a basal tear reference map, and modified to describe a fast, efficient and cost effective method of protein separation. The use of one pH 3-10 18 cm nonlinear immobilised pi-I gradient (IPG) strip and two mini-gels for the second-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) results in an effective separation of tear proteins which will be applied in diagnostic studies of tear samples.

Original languageEnglish
Pages (from-to)852-855
Number of pages4
JournalElectrophoresis
Volume19
Issue number5
DOIs
Publication statusPublished - 1998

Keywords

  • mass screening
  • mini-gel electrophoresis
  • tears
  • two-dimensional polyacrylamide gel electrophoresis

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