Development of new method for simultaneous analysis of piracetam and levetiracetam in pharmaceuticals and biological fluids

application in stability studies

Farhan Ahmed Siddiqui*, Nawab Sher, Nighat Shafi, Alisha Wafa Sial, Mansoor Ahmad, Mehjebeen, Huma Naseem

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)
1 Downloads (Pure)

Abstract

RP-HPLC ultraviolet detection simultaneous quantification of piracetam and levetiracetam has been developed and validated. The chromatography was obtained on a Nucleosil C18 column of 25 cm x 0.46 cm, 10 mu m, dimension. The mobile phase was a (70 : 30 v/v) mixture of 0.1 g/L of triethylamine and acetonitrile. Smooth flow of mobile phase at 1 mL/min was set and 205 nm wavelength was selected. Results were evaluated through statistical parameters which qualify the method reproducibility and selectivity for the quantification of piracetam, levetiracetam, and their impurities hence proving stability-indicating properties. The proposed method is significantly important, permitting the separation of the main constituent piracetam from levetiracetam. Linear behavior was observed between 20 ng/mL and 10000 ng/mL for both drugs. The proposed method was checked in bulk drugs, dosage formulations, physiological condition, and clinical investigations and excellent outcome was witnessed.

Original languageEnglish
Article number758283
Pages (from-to)1-8
Number of pages8
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • tandem mass-spectrometry
  • human plasma
  • liquid-chromatography
  • cerebrospinal-fluid
  • human serum
  • RP-HPLC
  • impurities
  • quantification
  • injection

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