TY - JOUR
T1 - Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae α-amylase in industrial strains of Saccharomyces cerevisiae
AU - Gundllapalli Moses, S. B.
AU - Cordero Otero, R. R.
AU - La Grange, D. C.
AU - Van Rensburg, P.
AU - Pretorius, I. S.
PY - 2002
Y1 - 2002
N2 - A haploid laboratory strain and four industrial (baking, brewing, wine, ATCC) strains of Saccharomyces cerevisiae were transformed with the Lipomyces kononenkoae α-amylase-encoding gene (LKA1). These transformants displayed significant differences in terms of the level of secretory expression of LKA1 under control of the PGK1 promoter and terminator, as well as their ability to produce and secrete the LKA1-encoded rawstarch-degrading α-amylase and to ferment starch. These results demonstrate the importance of the selection of appropriate host strains for yeast development pursuant to starch conversion into commercially important commodities via consolidated bioprocessing.
AB - A haploid laboratory strain and four industrial (baking, brewing, wine, ATCC) strains of Saccharomyces cerevisiae were transformed with the Lipomyces kononenkoae α-amylase-encoding gene (LKA1). These transformants displayed significant differences in terms of the level of secretory expression of LKA1 under control of the PGK1 promoter and terminator, as well as their ability to produce and secrete the LKA1-encoded rawstarch-degrading α-amylase and to ferment starch. These results demonstrate the importance of the selection of appropriate host strains for yeast development pursuant to starch conversion into commercially important commodities via consolidated bioprocessing.
UR - http://www.scopus.com/inward/record.url?scp=0036242027&partnerID=8YFLogxK
U2 - 10.1023/A:1015003610472
DO - 10.1023/A:1015003610472
M3 - Article
VL - 24
SP - 651
EP - 656
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 8
ER -