Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae α-amylase in industrial strains of Saccharomyces cerevisiae

S. B. Gundllapalli Moses; R. R. Cordero Otero; D. C. La Grange; P. Van Rensburg; I. S. Pretorius

doi:10.1023/A:1015003610472

Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae α-amylase in industrial strains of Saccharomyces cerevisiae

S. B. Gundllapalli Moses, R. R. Cordero Otero, D. C. La Grange, P. Van Rensburg, I. S. Pretorius^*

^*Corresponding author for this work

Research output: Contribution to journal › Article

18 Citations (Scopus)

Abstract

A haploid laboratory strain and four industrial (baking, brewing, wine, ATCC) strains of Saccharomyces cerevisiae were transformed with the Lipomyces kononenkoae α-amylase-encoding gene (LKA1). These transformants displayed significant differences in terms of the level of secretory expression of LKA1 under control of the PGK1 promoter and terminator, as well as their ability to produce and secrete the LKA1-encoded rawstarch-degrading α-amylase and to ferment starch. These results demonstrate the importance of the selection of appropriate host strains for yeast development pursuant to starch conversion into commercially important commodities via consolidated bioprocessing.

Original language	English
Pages (from-to)	651-656
Number of pages	6
Journal	Biotechnology Letters
Volume	24
Issue number	8
DOIs	https://doi.org/10.1023/A:1015003610472
Publication status	Published - 2002
Externally published	Yes

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Gundllapalli Moses, S. B., Cordero Otero, R. R., La Grange, D. C., Van Rensburg, P., & Pretorius, I. S. (2002). Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae α-amylase in industrial strains of Saccharomyces cerevisiae. Biotechnology Letters, 24(8), 651-656. https://doi.org/10.1023/A:1015003610472

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abstract = "A haploid laboratory strain and four industrial (baking, brewing, wine, ATCC) strains of Saccharomyces cerevisiae were transformed with the Lipomyces kononenkoae α-amylase-encoding gene (LKA1). These transformants displayed significant differences in terms of the level of secretory expression of LKA1 under control of the PGK1 promoter and terminator, as well as their ability to produce and secrete the LKA1-encoded rawstarch-degrading α-amylase and to ferment starch. These results demonstrate the importance of the selection of appropriate host strains for yeast development pursuant to starch conversion into commercially important commodities via consolidated bioprocessing.",

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Different genetic backgrounds influence the secretory expression of the LKA1-encoded Lipomyces kononenkoae α-amylase in industrial strains of Saccharomyces cerevisiae. / Gundllapalli Moses, S. B.; Cordero Otero, R. R.; La Grange, D. C.; Van Rensburg, P.; Pretorius, I. S.

In: Biotechnology Letters, Vol. 24, No. 8, 2002, p. 651-656.

Research output: Contribution to journal › Article

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AU - Gundllapalli Moses, S. B.

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