TY - JOUR
T1 - Differential accumulation of paralytic shellfish toxins from Alexandrium minutum in the pearl oyster, Pinctada imbricata
AU - Murray, Shauna A.
AU - O'Connor, Wayne A.
AU - Alvin, Alfonsus
AU - Mihali, Troco K.
AU - Kalaitzis, John
AU - Neilan, Brett A.
PY - 2009/9/1
Y1 - 2009/9/1
N2 - To investigate the potential for differential accumulation of paralytic shellfish toxins (PSTs) in various tissues of the akoya pearl oyster, Pinctada imbricata, two feeding trials were carried out using the PST-producing dinoflagellate, Alexandrium minutum. When fed with A. minutum at concentrations between 100 and 1300 cells ml-1, the maximum clearance by P. imbricata was shown to occur at a density of 300 cells ml-1. When fed twice daily at this rate for up 12 days, P. imbricata accumulated analogues of gonyautoxins (GTXs): GTXs 1,4 and 2,3. The levels of GTXs in the viscera increased progressively on days 4, 8 and 12 to peak at 17.9 ± 4.47 μg STX-equivalent 100 g-1 biomass. Following 12 days of depuration, in the absence of A. minutum, GTX levels fell by approximately 65% to 6.0 ± 2.20 μg STX-equivalent 100 g-1 biomass. No GTX was found in the oysters at the start of the trial or in untreated controls. The accumulation of GTX was found to be tissue specific. No GTX was detected in the muscle tissue of P. imbricata during the feeding trial.
AB - To investigate the potential for differential accumulation of paralytic shellfish toxins (PSTs) in various tissues of the akoya pearl oyster, Pinctada imbricata, two feeding trials were carried out using the PST-producing dinoflagellate, Alexandrium minutum. When fed with A. minutum at concentrations between 100 and 1300 cells ml-1, the maximum clearance by P. imbricata was shown to occur at a density of 300 cells ml-1. When fed twice daily at this rate for up 12 days, P. imbricata accumulated analogues of gonyautoxins (GTXs): GTXs 1,4 and 2,3. The levels of GTXs in the viscera increased progressively on days 4, 8 and 12 to peak at 17.9 ± 4.47 μg STX-equivalent 100 g-1 biomass. Following 12 days of depuration, in the absence of A. minutum, GTX levels fell by approximately 65% to 6.0 ± 2.20 μg STX-equivalent 100 g-1 biomass. No GTX was found in the oysters at the start of the trial or in untreated controls. The accumulation of GTX was found to be tissue specific. No GTX was detected in the muscle tissue of P. imbricata during the feeding trial.
KW - Paralytic shellfish toxins
KW - Pearl oyster
KW - Pinctada
KW - Saxitoxin
UR - http://www.scopus.com/inward/record.url?scp=67649400366&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2009.04.005
DO - 10.1016/j.toxicon.2009.04.005
M3 - Article
C2 - 19375444
AN - SCOPUS:67649400366
VL - 54
SP - 217
EP - 223
JO - Toxicon
JF - Toxicon
SN - 0041-0101
IS - 3
ER -