Differential metabolic response of cultured rice (Oryza sativa) cells exposed to high- And low-temperature stress

Chumithri Gayani Gammulla, Dana Pascovici, Brian J. Atwell, Paul A. Haynes

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Global mean temperatures are expected to rise by 2-4.5°C by 2100, accompanied by an increase in frequency and amplitude of extreme temperature events. Greater climatic extremes and an expanded range of cultivation will expose rice to increasing stress in the future. Understanding gene expression in disparate thermal regimes is important for the engineering of cultivars with tolerance to nonoptimal temperatures. Our study investigated the proteomic responses of rice cell suspension cultures to sudden temperature changes. Cell cultures grown at 28°C were subjected to 3-day exposure to 12 or 20°C for low-temperature stress, and 36 or 44°C for high-temperature stress. Quantitative label-free shotgun proteomic analysis was performed on biological triplicates of each treatment. Over 1900 proteins were expressed in one or more temperature treatments, and, of these, more than 850 were found to be responsive to either of the temperature extremes. These temperature-responsive proteins included more than 300 proteins which were uniquely expressed at either 12 or 44°C. Our study also identified 40 novel stress-response proteins and observed that switching between the classical and the alternative pathways of sucrose metabolism occurs in response to extremes of temperature.

LanguageEnglish
Pages3001-3019
Number of pages19
JournalProteomics
Volume10
Issue number16
DOIs
Publication statusPublished - Aug 2010

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Temperature
Cell culture
Proteomics
Proteins
Cell Culture Techniques
Oryza
Firearms
Heat-Shock Proteins
Metabolism
Gene expression
Sucrose
Labels
Suspensions
Hot Temperature
Gene Expression

Cite this

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title = "Differential metabolic response of cultured rice (Oryza sativa) cells exposed to high- And low-temperature stress",
abstract = "Global mean temperatures are expected to rise by 2-4.5°C by 2100, accompanied by an increase in frequency and amplitude of extreme temperature events. Greater climatic extremes and an expanded range of cultivation will expose rice to increasing stress in the future. Understanding gene expression in disparate thermal regimes is important for the engineering of cultivars with tolerance to nonoptimal temperatures. Our study investigated the proteomic responses of rice cell suspension cultures to sudden temperature changes. Cell cultures grown at 28°C were subjected to 3-day exposure to 12 or 20°C for low-temperature stress, and 36 or 44°C for high-temperature stress. Quantitative label-free shotgun proteomic analysis was performed on biological triplicates of each treatment. Over 1900 proteins were expressed in one or more temperature treatments, and, of these, more than 850 were found to be responsive to either of the temperature extremes. These temperature-responsive proteins included more than 300 proteins which were uniquely expressed at either 12 or 44°C. Our study also identified 40 novel stress-response proteins and observed that switching between the classical and the alternative pathways of sucrose metabolism occurs in response to extremes of temperature.",
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Differential metabolic response of cultured rice (Oryza sativa) cells exposed to high- And low-temperature stress. / Gammulla, Chumithri Gayani; Pascovici, Dana; Atwell, Brian J.; Haynes, Paul A.

In: Proteomics, Vol. 10, No. 16, 08.2010, p. 3001-3019.

Research output: Contribution to journalArticleResearchpeer-review

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