Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction

Helen Rizos*, Linda V. Gunn, Ray D. Pares, Michael R. Gillings

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    82 Citations (Scopus)

    Abstract

    A procedure based on the polymerase chain reaction (PCR) has been developed to classify cucumber mosaic cucumovirus (CMV) isolates accurately into two subgroups. Two CMV-specific primers that flank the CMV capsid protein gene were used to amplify a DNA fragment of approximately 870 bp. Restriction enzyme analysis of this fragment produces distinct restriction patterns that assign the CMV isolate into one of two subgroups. These two restriction groups correlate with the previously established CMV subgroupings; this PCR-based method may provide a simple alternative to the serological assays used for typing CMV isolates.

    Original languageEnglish
    Pages (from-to)2099-2103
    Number of pages5
    JournalJournal of General Virology
    Volume73
    Issue number8
    DOIs
    Publication statusPublished - 1992

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