Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction

Helen Rizos*, Linda V. Gunn, Ray D. Pares, Michael R. Gillings

*Corresponding author for this work

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

A procedure based on the polymerase chain reaction (PCR) has been developed to classify cucumber mosaic cucumovirus (CMV) isolates accurately into two subgroups. Two CMV-specific primers that flank the CMV capsid protein gene were used to amplify a DNA fragment of approximately 870 bp. Restriction enzyme analysis of this fragment produces distinct restriction patterns that assign the CMV isolate into one of two subgroups. These two restriction groups correlate with the previously established CMV subgroupings; this PCR-based method may provide a simple alternative to the serological assays used for typing CMV isolates.

Original languageEnglish
Pages (from-to)2099-2103
Number of pages5
JournalJournal of General Virology
Volume73
Issue number8
DOIs
Publication statusPublished - 1992

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