Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus

T. S. Lawson; R. E. Connally; S. Vemulpad; J. A. Piper

doi:10.1111/j.1472-765X.2011.03197.x

Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus

T. S. Lawson^*, R. E. Connally, S. Vemulpad, J. A. Piper

^*Corresponding author for this work

Research output: Contribution to journal › Comment/opinion

13 Citations (Scopus)

Abstract

Aims: To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and urea-NaCl reagents. Methods and Results: Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea-NaCl reagents on a hot-plate with a precise temperature control and identified with specific DNA probes. Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1h with a novel FISH method that used a single hot-plate and in the absence of dimethyl formamide. Significance and Impact of Study: A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.

Original language	English
Pages (from-to)	263-266
Number of pages	4
Journal	Letters in Applied Microbiology
Volume	54
Issue number	3
DOIs	https://doi.org/10.1111/j.1472-765X.2011.03197.x
Publication status	Published - Mar 2012

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title = "Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus",

abstract = "Aims: To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and urea-NaCl reagents. Methods and Results: Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea-NaCl reagents on a hot-plate with a precise temperature control and identified with specific DNA probes. Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1h with a novel FISH method that used a single hot-plate and in the absence of dimethyl formamide. Significance and Impact of Study: A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.",

author = "Lawson, {T. S.} and Connally, {R. E.} and S. Vemulpad and Piper, {J. A.}",

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doi = "10.1111/j.1472-765X.2011.03197.x",

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T1 - Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus

AU - Lawson, T. S.

AU - Connally, R. E.

AU - Vemulpad, S.

AU - Piper, J. A.

PY - 2012/3

Y1 - 2012/3

N2 - Aims: To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and urea-NaCl reagents. Methods and Results: Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea-NaCl reagents on a hot-plate with a precise temperature control and identified with specific DNA probes. Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1h with a novel FISH method that used a single hot-plate and in the absence of dimethyl formamide. Significance and Impact of Study: A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.

AB - Aims: To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and urea-NaCl reagents. Methods and Results: Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea-NaCl reagents on a hot-plate with a precise temperature control and identified with specific DNA probes. Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1h with a novel FISH method that used a single hot-plate and in the absence of dimethyl formamide. Significance and Impact of Study: A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.

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U2 - 10.1111/j.1472-765X.2011.03197.x

DO - 10.1111/j.1472-765X.2011.03197.x

M3 - Comment/opinion

VL - 54

SP - 263

EP - 266

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

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