Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus

T. S. Lawson*, R. E. Connally, S. Vemulpad, J. A. Piper

*Corresponding author for this work

    Research output: Contribution to journalComment/opinion

    13 Citations (Scopus)

    Abstract

    Aims: To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and urea-NaCl reagents. Methods and Results: Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea-NaCl reagents on a hot-plate with a precise temperature control and identified with specific DNA probes. Conclusions: Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1h with a novel FISH method that used a single hot-plate and in the absence of dimethyl formamide. Significance and Impact of Study: A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.

    Original languageEnglish
    Pages (from-to)263-266
    Number of pages4
    JournalLetters in Applied Microbiology
    Volume54
    Issue number3
    DOIs
    Publication statusPublished - Mar 2012

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