Direct evidence for the presence of two external NAD(P)H dehydrogenases coupled to the electron transport chain in plant mitochondria

Thomas H. Roberts, Kenneth M. Fredlund, Ian M. Møller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

Exogenous NADPH oxidation by purified mitochondria from both potato tuber and Arum maculatum spadix was completely and irreversibly inhibited by sub-micromolar diphenyleneiodonium (DPI), while exogenous NADH oxidation was inhibited to only a small degree. Addition of DPI caused the collapse of the membrane potential generated by NADPH oxidation, while the potential generated by NADH was unaffected. We conclude that there are two distint enzymes on the outer surface of the inner membrane of plant mitochondria, one specific for NADH, the other relatively specific for NADPH, with both enzymes linked to the electron transport chain.

Original languageEnglish
Pages (from-to)307-309
Number of pages3
JournalFEBS Letters
Volume373
Issue number3
DOIs
Publication statusPublished - 16 Oct 1995

Keywords

  • Diphenyleneiodonium
  • Electron transport chain
  • Mitochondria, plant
  • NAD(P)H dehydrogenase

Fingerprint

Dive into the research topics of 'Direct evidence for the presence of two external NAD(P)H dehydrogenases coupled to the electron transport chain in plant mitochondria'. Together they form a unique fingerprint.

Cite this