Discovery and heterologous biosynthesis of the burnettramic acids: rare PKS-NRPS-derived bolaamphiphilic pyrrolizidinediones from an Australian fungus, Aspergillus burnettii

Hang Li, Cameron L. M. Gilchrist, Heather J. Lacey, Andrew Crombie, Daniel Vuong, John I. Pitt, Ernest Lacey, Yit-Heng Chooi, Andrew M. Piggott

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The burnettramic acids are a new class of antibiotics from an Australian fungus Aspergillus burnettii. The rare bolaamphiphilic scaffold consists of β-d-mannose linked to a pyrrolizidinedione unit via a 26-carbon chain. The most abundant metabolite displayed potent in vitro antifungal activity. Comparative genomics identified the hybrid PKS-NRPS bua gene cluster, which was verified by heterologous pathway reconstitution in Aspergillus nidulans.

LanguageEnglish
Pages1287-1291
Number of pages5
JournalOrganic Letters
Volume21
Issue number5
DOIs
Publication statusPublished - 8 Feb 2019

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Aspergillus
Aspergillus nidulans
biosynthesis
fungi
Biosynthesis
Mannose
Multigene Family
Genomics
Fungi
Carbon
Anti-Bacterial Agents
acids
Acids
antibiotics
metabolites
Scaffolds (biology)
Metabolites
genes
Genes
carbon

Cite this

Li, Hang ; Gilchrist, Cameron L. M. ; Lacey, Heather J. ; Crombie, Andrew ; Vuong, Daniel ; Pitt, John I. ; Lacey, Ernest ; Chooi, Yit-Heng ; Piggott, Andrew M. / Discovery and heterologous biosynthesis of the burnettramic acids : rare PKS-NRPS-derived bolaamphiphilic pyrrolizidinediones from an Australian fungus, Aspergillus burnettii. In: Organic Letters. 2019 ; Vol. 21, No. 5. pp. 1287-1291.
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abstract = "The burnettramic acids are a new class of antibiotics from an Australian fungus Aspergillus burnettii. The rare bolaamphiphilic scaffold consists of β-d-mannose linked to a pyrrolizidinedione unit via a 26-carbon chain. The most abundant metabolite displayed potent in vitro antifungal activity. Comparative genomics identified the hybrid PKS-NRPS bua gene cluster, which was verified by heterologous pathway reconstitution in Aspergillus nidulans.",
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Discovery and heterologous biosynthesis of the burnettramic acids : rare PKS-NRPS-derived bolaamphiphilic pyrrolizidinediones from an Australian fungus, Aspergillus burnettii. / Li, Hang; Gilchrist, Cameron L. M.; Lacey, Heather J.; Crombie, Andrew; Vuong, Daniel; Pitt, John I.; Lacey, Ernest; Chooi, Yit-Heng; Piggott, Andrew M.

In: Organic Letters, Vol. 21, No. 5, 08.02.2019, p. 1287-1291.

Research output: Contribution to journalArticleResearchpeer-review

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