Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: a comparative study

Piklu Roy Chowdhury, Ana Ingold, Natasha Vanegas, Elena Martínez, John Merlino, Andrea Karina Merkier, Mercedes Castro, Gerardo González Rocha, Graciela Borthagaray, Daniela Centrón, Helia Bello Toledo, Carolina M. Márquez, H. W. Stokes

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A comparative genetic analysis of 42 clinical Klebsiella pneumoniae isolates, resistant to two or more antibiotics belonging to the broad-spectrum β-lactam group, sourced from Sydney, Australia, and three South American countries is presented. The study focuses on the genetic contexts of class 1 integrons, mobilizable genetic elements best known for their role in the rapid evolution of antibiotic resistance among Gram-negative pathogens. It was found that the class 1 integrons in this cohort were located in a number of different genetic contexts with clear regional differences. In Sydney, IS26-associated Tn21-like transposons on IncL/M plasmids contribute greatly to the dispersal of integron-associated multiple-drug-resistant (MDR) loci. In contrast, in the South American countries, Tn1696-like transposons on an IncA/C plasmid(s) appeared to be disseminating a characteristic MDR region. A range of mobile genetic elements is clearly being recruited by clinically important mobile class 1 integrons, and these elements appear to be becoming more common with time. This in turn is driving the evolution of complex and laterally mobile MDR units and may further complicate antibiotic therapy.

LanguageEnglish
Pages3140-3149
Number of pages10
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number7
DOIs
Publication statusPublished - Jul 2011

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Integrons
Klebsiella pneumoniae
Multiple Drug Resistance
Genes
Plasmids
Pharmaceutical Preparations
Interspersed Repetitive Sequences
Anti-Bacterial Agents
South Australia
beta-Lactams
Microbial Drug Resistance

Cite this

Chowdhury, Piklu Roy ; Ingold, Ana ; Vanegas, Natasha ; Martínez, Elena ; Merlino, John ; Merkier, Andrea Karina ; Castro, Mercedes ; Rocha, Gerardo González ; Borthagaray, Graciela ; Centrón, Daniela ; Toledo, Helia Bello ; Márquez, Carolina M. ; Stokes, H. W. / Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries : a comparative study. In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 7. pp. 3140-3149.
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abstract = "A comparative genetic analysis of 42 clinical Klebsiella pneumoniae isolates, resistant to two or more antibiotics belonging to the broad-spectrum β-lactam group, sourced from Sydney, Australia, and three South American countries is presented. The study focuses on the genetic contexts of class 1 integrons, mobilizable genetic elements best known for their role in the rapid evolution of antibiotic resistance among Gram-negative pathogens. It was found that the class 1 integrons in this cohort were located in a number of different genetic contexts with clear regional differences. In Sydney, IS26-associated Tn21-like transposons on IncL/M plasmids contribute greatly to the dispersal of integron-associated multiple-drug-resistant (MDR) loci. In contrast, in the South American countries, Tn1696-like transposons on an IncA/C plasmid(s) appeared to be disseminating a characteristic MDR region. A range of mobile genetic elements is clearly being recruited by clinically important mobile class 1 integrons, and these elements appear to be becoming more common with time. This in turn is driving the evolution of complex and laterally mobile MDR units and may further complicate antibiotic therapy.",
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Chowdhury, PR, Ingold, A, Vanegas, N, Martínez, E, Merlino, J, Merkier, AK, Castro, M, Rocha, GG, Borthagaray, G, Centrón, D, Toledo, HB, Márquez, CM & Stokes, HW 2011, 'Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: a comparative study', Antimicrobial Agents and Chemotherapy, vol. 55, no. 7, pp. 3140-3149. https://doi.org/10.1128/AAC.01529-10

Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries : a comparative study. / Chowdhury, Piklu Roy; Ingold, Ana; Vanegas, Natasha; Martínez, Elena; Merlino, John; Merkier, Andrea Karina; Castro, Mercedes; Rocha, Gerardo González; Borthagaray, Graciela; Centrón, Daniela; Toledo, Helia Bello; Márquez, Carolina M.; Stokes, H. W.

In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 7, 07.2011, p. 3140-3149.

Research output: Contribution to journalArticleResearchpeer-review

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