Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections

Timothy J. Green, Agnes Vergnes, Caroline Montagnani*, Julien De Lorgeril

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)
17 Downloads (Pure)

Abstract

Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host.

Original languageEnglish
Article number72
Pages (from-to)1-11
Number of pages11
JournalVeterinary Research
Volume47
DOIs
Publication statusPublished - 21 Jul 2016

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Fingerprint

Dive into the research topics of 'Distinct immune responses of juvenile and adult oysters (<i>Crassostrea gigas</i>) to viral and bacterial infections'. Together they form a unique fingerprint.

Cite this