Distinctive autofluorescence of urine samples from individuals with bacteriuria compared with normals

Ayad G. Anwer; Perinchery M. Sandeep; Ewa M. Goldys; Subramanyam Vemulpad

doi:10.1016/j.cca.2008.11.021

Distinctive autofluorescence of urine samples from individuals with bacteriuria compared with normals

Ayad G. Anwer, Perinchery M. Sandeep, Ewa M. Goldys, Subramanyam Vemulpad^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Background: A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes. Method: This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria. Results: Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group. Conclusion: The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.

Original language	English
Pages (from-to)	73-75
Number of pages	3
Journal	Clinica Chimica Acta
Volume	401
Issue number	1-2
DOIs	https://doi.org/10.1016/j.cca.2008.11.021
Publication status	Published - Mar 2009

Keywords

autofluorescence
human urine
bacteriuria
urinary tract infection
diagnostic tests
IDO

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10.1016/j.cca.2008.11.021

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title = "Distinctive autofluorescence of urine samples from individuals with bacteriuria compared with normals",

abstract = "Background: A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes. Method: This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria. Results: Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group. Conclusion: The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.",

keywords = "autofluorescence, human urine, bacteriuria, urinary tract infection, diagnostic tests, IDO",

author = "Anwer, {Ayad G.} and Sandeep, {Perinchery M.} and Goldys, {Ewa M.} and Subramanyam Vemulpad",

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AU - Anwer, Ayad G.

AU - Sandeep, Perinchery M.

AU - Goldys, Ewa M.

AU - Vemulpad, Subramanyam

PY - 2009/3

Y1 - 2009/3

N2 - Background: A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes. Method: This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria. Results: Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group. Conclusion: The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.

AB - Background: A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes. Method: This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria. Results: Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group. Conclusion: The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.

KW - autofluorescence

KW - human urine

KW - bacteriuria

KW - urinary tract infection

KW - diagnostic tests

KW - IDO

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JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 1-2

ER -

Distinctive autofluorescence of urine samples from individuals with bacteriuria compared with normals

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Keywords

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