Distribution and development of molecularly distinct perineuronal nets in visual thalamus

Ubadah Sabbagh, Aboozar Monavarfeshani, Kaiwen Su, Masoud Zabet-Moghadam, James Cole, Eric Carnival, Jianmin Su, Mehdi Mirzaei, Vivek Gupta, Ghasem Hosseini Salekdeh*, Michael A. Fox

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Visual information is detected by the retina and transmitted into the brain by retinal ganglion cells. In rodents, the visual thalamus is a major recipient of retinal ganglion cells axons and is divided into three functionally distinct nuclei: the dorsal lateral geniculate nucleus (dLGN), ventral LGN (vLGN), and intergeniculate leaflet. Despite being densely innervated by retinal input, each nucleus in rodent visual thalamus possesses diverse molecular profiles which underpin their unique circuitry and cytoarchitecture. Here, we combined large-scale unbiased proteomic and transcriptomic analyses to elucidate the molecular expression profiles of the developing mouse dLGN and vLGN. We identified several extracellular matrix proteins as differentially expressed in these regions, particularly constituent molecules of perineuronal nets (PNNs). Remarkably, we discovered at least two types of molecularly distinct Aggrecan-rich PNN populations in vLGN, exhibiting non-overlapping spatial, temporal, and cell-type specific expression patterns. The mechanisms responsible for the formation of these two populations of PNNs also differ as the formation of Cat315+ PNNs (but not WFA+ PNNs) required input from the retina. This study is first to suggest that cell type- and molecularly specific supramolecular assemblies of extracellular matrix may play important roles in the circuitry associated with the subcortical visual system and in the processing of visual information.

Original languageEnglish
Pages (from-to)626-646
Number of pages21
JournalJournal of Neurochemistry
Volume147
Issue number5
Early online date16 Oct 2018
DOIs
Publication statusPublished - Dec 2018

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Keywords

  • aggrecan
  • LGN
  • perineuronal net
  • retinogeniculate
  • thalamus
  • visual

Cite this

Sabbagh, U., Monavarfeshani, A., Su, K., Zabet-Moghadam, M., Cole, J., Carnival, E., ... Fox, M. A. (2018). Distribution and development of molecularly distinct perineuronal nets in visual thalamus. Journal of Neurochemistry, 147(5), 626-646. https://doi.org/10.1111/jnc.14614