DNA binding and bending by dinuclear complexes comprising ruthenium polypyridyl centres linked by a bis(pyridylimine) ligand

Ursula McDonnell, Matthew R. Hicks, Michael J. Hannon, Alison Rodger*

*Corresponding author for this work

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The interaction of enantiomerically pure dinuclear complexes of the form [Ru2(L-L)4L1]4+ (where L-L = 2,2-bipyridine (bpy) or 1,10-phenanthroline (phen) and L1 = bis(pyridylimine) ligand ((C5H4N)C{double bond, long}N(C6H4))2CH2)) with ct-DNA have been investigated by absorbance, circular dichroism, fluorescence displacement assays, thermal analysis, linear dichroism and gel electrophoresis. The complexes all bind more strongly to DNA than ethidium bromide, stabilise DNA and have a significant bending effect on DNA. The data for Δ,Δ-[Ru2(bpy)4L1]4+ are consistent with it binding to DNA outside the grooves wrapping the DNA about it. By way of contrast the other complexes are groove-binders. The phen complexes provide a chemically and enantiomerically stable alternative to the DNA-coiling di-iron triple-helical cylinder previously studied. In contrast to the di-iron helicates, the phen complexes show DNA sequence effects with Δ,Δ-[Ru2(phen)4L1]4+ binding preferentially to GC and Λ,Λ-[Ru2(phen)4L1]4+ to AT.

Original languageEnglish
Pages (from-to)2052-2059
Number of pages8
JournalJournal of Inorganic Biochemistry
Volume102
Issue number12
DOIs
Publication statusPublished - Dec 2008
Externally publishedYes

Keywords

  • Bimetallo complexes
  • DNA bending
  • DNA binding
  • Linear dichroism
  • Ruthenium metal complexes

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