DNA-drug systems

how circular dichroism data is complemented by other spectroscopic techniques

Alison Rodger*, Harriet C. Latham, Paul Wormell, Andrew Parkinson, Matthew Ismail, Karen J. Sanders

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Circular dichroism (CD) measurements of DNA-drug complexes of two different systems are interpreted with the aid of other experimental and calculation data. The induced CD (ICD) of the polyamine mimic [Co(NH3)]3+ has been used to determine the low drug:DNA ratio binding constant with calf thymus DNA of 1,00,000 M-1 at 11 mM Na+. The site size is large. Linear dichroism (LD) shows how the DNA bends in accord with changes in DNA conformation observed in the CD. 9-hydroxyelliptine DNA binding in the intercalation limit and the higher concentration mode that gives rise to exciton CD signals have been probed using normal absorption, LD, both film and flow, and resonance light scattering. CNDO/S-CI calculations have also been investigated as a means of determining transition moments and intensities of individual transitions in a complex overlapping spectrum.

Original languageEnglish
Pages (from-to)395-408
Number of pages14
JournalEnantiomer
Volume3
Issue number4-5
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • 9-Hydroxyellipticine
  • Absorbance
  • Circular dichroism
  • Cobalt hexammine
  • DNA
  • Linear dichroism
  • Resonance light scattering
  • Spermine

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    Rodger, A., Latham, H. C., Wormell, P., Parkinson, A., Ismail, M., & Sanders, K. J. (1998). DNA-drug systems: how circular dichroism data is complemented by other spectroscopic techniques. Enantiomer, 3(4-5), 395-408.