DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC)

Bing Yu, Nicole Sawyer, Christine Chiu, Peter J. Oefner, Peter A. Underhill

Research output: Chapter in Book/Report/Conference proceedingChapter

25 Citations (Scopus)

Abstract

DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.
Original languageEnglish
Title of host publicationCurrent Protocols in Human Genetics
PublisherJohn Wiley & Sons
Pages1-14
Number of pages14
ISBN (Electronic)9780471142904
DOIs
Publication statusPublished - 2006
Externally publishedYes

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