DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC)

Bing Yu; Nicole Sawyer; Christine Chiu; Peter J. Oefner; Peter A. Underhill

doi:10.1002/0471142905.hg0710s48

DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC)

Bing Yu, Nicole Sawyer, Christine Chiu, Peter J. Oefner, Peter A. Underhill

Research output: Chapter in Book/Report/Conference proceeding › Chapter

29 Citations (Scopus)

Abstract

DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.

Original language	English
Title of host publication	Current Protocols in Human Genetics
Publisher	John Wiley & Sons
Pages	1-14
Number of pages	14
ISBN (Electronic)	9780471142904
DOIs	https://doi.org/10.1002/0471142905.hg0710s48
Publication status	Published - 2006
Externally published	Yes

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10.1002/0471142905.hg0710s48

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abstract = "DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.",

author = "Bing Yu and Nicole Sawyer and Christine Chiu and Oefner, {Peter J.} and Underhill, {Peter A.}",

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AU - Chiu, Christine

AU - Oefner, Peter J.

AU - Underhill, Peter A.

PY - 2006

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AB - DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.

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BT - Current Protocols in Human Genetics

PB - John Wiley & Sons

ER -

DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC)

Abstract

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