DNA nanoclew templated spherical nucleic acids for siRNA delivery

Weimin Ruan, Meng Zheng, Yang An, Yuanyuan Liu, David Lovejoy, Mingcong Hao, Yan Zou, Albert Lee, Shu Yang, Yiqing Lu, Marco Morsch, Roger Chung, Bingyang Shi

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostucture we have termed a DNA nanoclew (DC). After uptake by cultured cancer cells, these SNA nanoparticles release engrafted siRNAs by cleavage of intracellular Dicer enzyme. Moreover, in vitro expriments display that such SNAs demonstrate potent gene knockdown at both miRNA and protein levels, while with negligible cytotoxicity.
LanguageEnglish
Pages3609-3612
Number of pages4
JournalChemical Engineering Communications
Volume54
DOIs
Publication statusPublished - 14 Apr 2018

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Nucleic acids
Nucleic Acids
Small Interfering RNA
DNA
Ribonuclease III
Cytotoxicity
MicroRNAs
Enzymes
Genes
Cells
Nanoparticles
Proteins

Cite this

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title = "DNA nanoclew templated spherical nucleic acids for siRNA delivery",
abstract = "A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostucture we have termed a DNA nanoclew (DC). After uptake by cultured cancer cells, these SNA nanoparticles release engrafted siRNAs by cleavage of intracellular Dicer enzyme. Moreover, in vitro expriments display that such SNAs demonstrate potent gene knockdown at both miRNA and protein levels, while with negligible cytotoxicity.",
author = "Weimin Ruan and Meng Zheng and Yang An and Yuanyuan Liu and David Lovejoy and Mingcong Hao and Yan Zou and Albert Lee and Shu Yang and Yiqing Lu and Marco Morsch and Roger Chung and Bingyang Shi",
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DNA nanoclew templated spherical nucleic acids for siRNA delivery. / Ruan, Weimin; Zheng, Meng; An, Yang; Liu, Yuanyuan; Lovejoy, David; Hao, Mingcong; Zou, Yan; Lee, Albert; Yang, Shu; Lu, Yiqing; Morsch, Marco; Chung, Roger; Shi, Bingyang.

In: Chemical Engineering Communications, Vol. 54, 14.04.2018, p. 3609-3612.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Ruan, Weimin

AU - Zheng, Meng

AU - An, Yang

AU - Liu, Yuanyuan

AU - Lovejoy, David

AU - Hao, Mingcong

AU - Zou, Yan

AU - Lee, Albert

AU - Yang, Shu

AU - Lu, Yiqing

AU - Morsch, Marco

AU - Chung, Roger

AU - Shi, Bingyang

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DO - 10.1039/C7CC09257A

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