DNA nanoclew templated spherical nucleic acids for siRNA delivery

Weimin Ruan; Meng Zheng; Yang An; Yuanyuan Liu; David Lovejoy; Mingcong Hao; Yan Zou; Albert Lee; Shu Yang; Yiqing Lu; Marco Morsch; Roger Chung; Bingyang Shi

doi:10.1039/C7CC09257A

DNA nanoclew templated spherical nucleic acids for siRNA delivery

Weimin Ruan, Meng Zheng, Yang An, Yuanyuan Liu, David Lovejoy, Mingcong Hao, Yan Zou, Albert Lee, Shu Yang, Yiqing Lu, Marco Morsch, Roger Chung, Bingyang Shi

Macquarie University Centre for Motor Neuron Disease Research

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostucture we have termed a DNA nanoclew (DC). After uptake by cultured cancer cells, these SNA nanoparticles release engrafted siRNAs by cleavage of intracellular Dicer enzyme. Moreover, in vitro expriments display that such SNAs demonstrate potent gene knockdown at both miRNA and protein levels, while with negligible cytotoxicity.

Original language	English
Pages (from-to)	3609-3612
Number of pages	4
Journal	Chemical Communications
Volume	54
DOIs	https://doi.org/10.1039/C7CC09257A
Publication status	Published - 14 Apr 2018

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abstract = "A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostucture we have termed a DNA nanoclew (DC). After uptake by cultured cancer cells, these SNA nanoparticles release engrafted siRNAs by cleavage of intracellular Dicer enzyme. Moreover, in vitro expriments display that such SNAs demonstrate potent gene knockdown at both miRNA and protein levels, while with negligible cytotoxicity.",

author = "Weimin Ruan and Meng Zheng and Yang An and Yuanyuan Liu and David Lovejoy and Mingcong Hao and Yan Zou and Albert Lee and Shu Yang and Yiqing Lu and Marco Morsch and Roger Chung and Bingyang Shi",

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T1 - DNA nanoclew templated spherical nucleic acids for siRNA delivery

AU - Ruan, Weimin

AU - Zheng, Meng

AU - An, Yang

AU - Liu, Yuanyuan

AU - Lovejoy, David

AU - Hao, Mingcong

AU - Zou, Yan

AU - Lee, Albert

AU - Yang, Shu

AU - Lu, Yiqing

AU - Morsch, Marco

AU - Chung, Roger

AU - Shi, Bingyang

PY - 2018/4/14

Y1 - 2018/4/14

N2 - A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostucture we have termed a DNA nanoclew (DC). After uptake by cultured cancer cells, these SNA nanoparticles release engrafted siRNAs by cleavage of intracellular Dicer enzyme. Moreover, in vitro expriments display that such SNAs demonstrate potent gene knockdown at both miRNA and protein levels, while with negligible cytotoxicity.

AB - A superior biocompatible spherical nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the surface of a core composed of a spherical DNA nanostucture we have termed a DNA nanoclew (DC). After uptake by cultured cancer cells, these SNA nanoparticles release engrafted siRNAs by cleavage of intracellular Dicer enzyme. Moreover, in vitro expriments display that such SNAs demonstrate potent gene knockdown at both miRNA and protein levels, while with negligible cytotoxicity.

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DO - 10.1039/C7CC09257A

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SN - 1364-548X

VL - 54

SP - 3609

EP - 3612

JO - Chemical Communications

JF - Chemical Communications

ER -

DNA nanoclew templated spherical nucleic acids for siRNA delivery

Abstract

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