TY - JOUR
T1 - DNA origami-constructed nanotapes for sunitinib adsorption and inhibition of renal clear carcinoma cells
AU - Li, Lin
AU - Yao, Xuxiang
AU - Wei, Pengyao
AU - He, Dongdong
AU - Ding, Qiaojiao
AU - Bai, Bing
AU - Lv, Xiuyi
AU - Kuzuya, Akinori
AU - Wang, Yuling
AU - Wu, Kerong
AU - Wang, Kaizhe
AU - Zheng, Jianping
N1 - Copyright the Author(s) 2024. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2024
Y1 - 2024
N2 - Sunitinib (SUN) is a first-line drug for the treatment of renal clear carcinoma cells by targeting receptor tyrosine kinases (RTK) on the cell membrane. However, the effective delivery of SUN to the cell membrane remains a significant challenge. In this study, we fabricated precisely structured DNA nanotapes with strong surface SUN adhesion, enabling RTK inhibition of renal clear carcinoma cells. In our design, the precisely assembled linear topological six-helical-bundle DNA origami serves as the framework, and positively charged chitosan is adsorbed onto the DNA origami surface, thereby forming DNA nanotapes. The SUN was efficiently loaded onto the surface of the DNA nanotapes by electrostatic interaction. We found that DNA nanotapes exhibit excellent stability in serum. Importantly, DNA nanotapes carrying SUN can achieve prolonged cell membrane retention and inhibit RTK, thereby enhancing cytotoxicity toward 786-0 cells. Taken together, this study provides a promising candidate platform for the efficient delivery of cell membrane receptor inhibitors in anticancer therapy.
AB - Sunitinib (SUN) is a first-line drug for the treatment of renal clear carcinoma cells by targeting receptor tyrosine kinases (RTK) on the cell membrane. However, the effective delivery of SUN to the cell membrane remains a significant challenge. In this study, we fabricated precisely structured DNA nanotapes with strong surface SUN adhesion, enabling RTK inhibition of renal clear carcinoma cells. In our design, the precisely assembled linear topological six-helical-bundle DNA origami serves as the framework, and positively charged chitosan is adsorbed onto the DNA origami surface, thereby forming DNA nanotapes. The SUN was efficiently loaded onto the surface of the DNA nanotapes by electrostatic interaction. We found that DNA nanotapes exhibit excellent stability in serum. Importantly, DNA nanotapes carrying SUN can achieve prolonged cell membrane retention and inhibit RTK, thereby enhancing cytotoxicity toward 786-0 cells. Taken together, this study provides a promising candidate platform for the efficient delivery of cell membrane receptor inhibitors in anticancer therapy.
UR - http://www.scopus.com/inward/record.url?scp=85199765263&partnerID=8YFLogxK
U2 - 10.1021/acsomega.4c03091
DO - 10.1021/acsomega.4c03091
M3 - Article
C2 - 39130609
SN - 2470-1343
VL - 9
SP - 33765−33772
JO - ACS Omega
JF - ACS Omega
IS - 31
ER -