Dopamine detection at antifouling conical-tip carbon electrodes

Simona Baluchova*, Jan Klouda, Jiri Barek, Karolina Schwarzova-Peckova, Danny K. Y. Wong

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

    Abstract

    A significant achievement in this work is the development of antifouling conical-tip carbon electrodes (similar to 2.7 mu m tip diameter and similar to 165 mu m axial length) suitable for detection of the neurotransmitter dopamine in-vivo. These electrodes were hydrogenated using a diphenylsilane reduction method to yield a hydrophobic surface to deter adsorption of amphiphilic biomolecules. Initially, hydrogenated carbon electrodes were electrochemically characterised using several redox markers. The degree of antifouling was then assessed by the voltammetric signal change of dopamine at these electrodes before and after being incubated in a fouling solution containing bovine serum albumin, cytochrome C (both are proteins) and caproic acid (a lipid). In our work, we have obtained only a 6.9% (standard deviation 3.5%, N = 40) decrease in dopamine signals at the hydro-genated carbon electrodes. These results strongly support the diphenylsilane reduction strategy for the development of antifouling biosensors for dopamine detection in biological matrices.

    Original languageEnglish
    Title of host publicationProceedings of the 16th International Students Conference “Modern Analytical Chemistry”
    EditorsKarel Nesměrák
    Place of PublicationPrague
    PublisherCharles University
    Pages19-24
    Number of pages6
    ISBN (Print)9788074440793
    Publication statusPublished - 2020
    Event16th Annual International Students Conference on Modern Analytical Chemistry - Prague, Czech Republic
    Duration: 17 Sept 202018 Sept 2020

    Conference

    Conference16th Annual International Students Conference on Modern Analytical Chemistry
    Country/TerritoryCzech Republic
    CityPrague
    Period17/09/2018/09/20

    Keywords

    • antifouling electrodes
    • diphenylsilane reduction method
    • hydrogenated conical-tip carbon electrodes
    • voltammetric dopamine detection

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