Downregulation of insulin-like growth factor-1 receptor (IGF-1R) expression in human T lymphocyte activation

Roxana Schillaci, Mariana G. Brocardo, Adriana Galeano, Alicia Roldán

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Although it is known that IGF-1 increases T lymphocyte proliferation, the regulation of its receptor expression during the process has not been defined. Consequently the regulation of IGF-1R expression by IGF-1 and the activation events in human blood T lymphocytes and the Jurkat T cell line were now investigated. IGF-1R expression in nonstimulated Jurkat cells was confirmed and downregulation by IGF-1 was demonstrated. In addition, both cell types showed a time-dependent reduction in the number of IGF-1R- positive cells following activation, which was increased by IGF-1. In Jurkat cells the negative regulation of IGF-1R levels was correlated with the appearance and continuous increase in IL-2R. In T lymphocytes the decrease in IGF-1R expression was faster, reaching its plateau after 3 h of activation. Our findings suggest that loss of the IGF-1R is one of the initial events of the activation process not regulated by IL-2.

LanguageEnglish
Pages157-161
Number of pages5
JournalCellular Immunology
Volume183
Issue number2
DOIs
Publication statusPublished - 1 Feb 1998
Externally publishedYes

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Somatomedin Receptors
Lymphocyte Activation
Down-Regulation
T-Lymphocytes
Insulin-Like Growth Factor I
Jurkat Cells
Interleukin-2
Cell Line

Cite this

Schillaci, Roxana ; Brocardo, Mariana G. ; Galeano, Adriana ; Roldán, Alicia. / Downregulation of insulin-like growth factor-1 receptor (IGF-1R) expression in human T lymphocyte activation. In: Cellular Immunology. 1998 ; Vol. 183, No. 2. pp. 157-161.
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abstract = "Although it is known that IGF-1 increases T lymphocyte proliferation, the regulation of its receptor expression during the process has not been defined. Consequently the regulation of IGF-1R expression by IGF-1 and the activation events in human blood T lymphocytes and the Jurkat T cell line were now investigated. IGF-1R expression in nonstimulated Jurkat cells was confirmed and downregulation by IGF-1 was demonstrated. In addition, both cell types showed a time-dependent reduction in the number of IGF-1R- positive cells following activation, which was increased by IGF-1. In Jurkat cells the negative regulation of IGF-1R levels was correlated with the appearance and continuous increase in IL-2R. In T lymphocytes the decrease in IGF-1R expression was faster, reaching its plateau after 3 h of activation. Our findings suggest that loss of the IGF-1R is one of the initial events of the activation process not regulated by IL-2.",
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Downregulation of insulin-like growth factor-1 receptor (IGF-1R) expression in human T lymphocyte activation. / Schillaci, Roxana; Brocardo, Mariana G.; Galeano, Adriana; Roldán, Alicia.

In: Cellular Immunology, Vol. 183, No. 2, 01.02.1998, p. 157-161.

Research output: Contribution to journalArticleResearchpeer-review

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