Draxin, an axon guidance protein, affects chick trunk neural crest migration

Yuhong Su, Iftekhar Bin Naser, Shahidul M. Islam, Sanbing Zhang, Giasuddin Ahmed, Sandy Chen, Yohei Shinmyo, Minoru Kawakami, Ken-ichi Yamamura, Hideaki Tanaka*

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The neural crest is a multipotent population of migratory cells that arises in the central nervous system and subsequently migrates along defined stereotypic pathways. In the present work, we analyzed the role of a repulsive axon guidance protein, draxin, in the migration of neural crest cells. Draxin is expressed in the roof plate of the chick trunk spinal cord and around the early migration pathway of neural crest cells. Draxin modulates chick neural crest cell migration in vitro by reducing the polarization of these cells. When exposed to draxin, the velocity of migrating neural crest cells was reduced, and the cells changed direction so frequently that the net migration distance was also reduced. Overexpression of draxin also caused some early migrating neural crest cells to change direction to the dorsolateral pathway in the chick trunk region, presumably due to draxin's inhibitory activity. These results demonstrate that draxin, an axon guidance protein, can also affect trunk neural crest migration in the chick embryo.

Original languageEnglish
Pages (from-to)787-796
Number of pages10
JournalDevelopment Growth and Differentiation
Volume51
Issue number9
DOIs
Publication statusPublished - Dec 2009
Externally publishedYes

Keywords

  • cytoskeleton
  • draxin
  • guidance cue
  • inhibition
  • neural crest migration
  • MOUSE SPINAL-CORD
  • IN-SITU
  • SEGMENTAL MIGRATION
  • REPULSIVE GUIDANCE
  • CELL-MIGRATION
  • NERVOUS-SYSTEM
  • ROSTRAL HALF
  • MOTOR AXONS
  • RECEPTORS
  • LIGANDS

Cite this

Su, Y., Bin Naser, I., Islam, S. M., Zhang, S., Ahmed, G., Chen, S., ... Tanaka, H. (2009). Draxin, an axon guidance protein, affects chick trunk neural crest migration. Development Growth and Differentiation, 51(9), 787-796. https://doi.org/10.1111/j.1440-169X.2009.01137.x