Dual response surface-optimized synthesis of L-menthyl conjugated linoleate in solvent-free system by Candida rugosa lipase

Zhen Li, Yulun Wang, Jia Li, Ping Wang, Wei Wei, Yang Gao, Chenyin Fu, Wenqi Dong

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Lipase-catalyzed synthesis of L-menthyl conjugated linoleate in solvent-free system was studied in this paper. Duel response surface methodology was employed to explore the factors which would influence the reaction conversion by a range of independent experiments. The conditions of reaction temperature, reaction time, enzyme amount, substrate molar ratio and water content were symmetrically investigated. When the substrates were 1 mmol CLA and 1 mmol L-menthol, the maximum conversion (79.1+/-0.8%) was obtained at 30 degrees C, Candida rugosa lipase of 33.7% (w/w by weight of L-menthol), water content of 32% (w/w by weight of L-menthol), reaction time of 43 h. The product isomers (9Z,11E-/10E,12Z-=63/37) were analyzed by GC/MS. The similarity between the oleic acid and 9Z,11E-CLA conformations which were obtained by molecular modeling could account for the specific catalyzed by C. rugosa lipase.
Original languageEnglish
Pages (from-to)1305-1309
Number of pages5
JournalBioresource Technology
Volume101
Issue number4
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

Keywords

  • L-Menthyl conjugated linoleate
  • Lipase-catalyzed
  • Response surface methodology

Fingerprint Dive into the research topics of 'Dual response surface-optimized synthesis of L-menthyl conjugated linoleate in solvent-free system by Candida rugosa lipase'. Together they form a unique fingerprint.

Cite this