Early effects of insulin-like growth factor-1 in activated human T lymphocytes

M. G. Brocardo, R. Schillaci, A. Galeano, M. Radrizzani, V. White, A. González Guerrico, T. A. Santa-Coloma, A. Roldán

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This study evaluates the effects of insulin-like growth factor (IGF)-1 receptor (IGF-1R) down-regulation in stimulated T lymphocytes by investigating the expression of early activation proteins CD69, CD25, and interleukin (IL)-2. We found that IGF-1 does not modify CD69 expression but increases transcription and protein synthesis of CD25 and IL-2. The lowest level of IGF-1R detected after 15 min of activation suggested that the effects of IGF-1 occur at the initiation of cell activation. The activation of IGF-1R was confirmed by IGF-1R phosphorylation and increased phosphorylation of microtubule-associated protein kinase. We also detected the alternative IGF-1 transcripts Ea, with paracrine/autocrine regulation, and Eb, with endocrine regulation, in Jurkat cells and in quiescent T lymphocytes, and we detected IGF-1 protein in the culture medium after stimulation. These data suggest that the proliferative effects of IGF-1 on T lymphocytes include both autocrine/paracrine and endocrine processes.

LanguageEnglish
Pages297-305
Number of pages9
JournalJournal of Leukocyte Biology
Volume70
Issue number2
Publication statusPublished - 2001
Externally publishedYes

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Somatomedins
T-Lymphocytes
Interleukin-2
Phosphorylation
Somatomedin Receptors
Proteins
Jurkat Cells
Microtubule-Associated Proteins
Protein Kinases
Culture Media
Down-Regulation

Keywords

  • CD25
  • IGF-1 mRNA
  • IGF-1 receptor
  • IL-2
  • MAPK

Cite this

Brocardo, M. G., Schillaci, R., Galeano, A., Radrizzani, M., White, V., González Guerrico, A., ... Roldán, A. (2001). Early effects of insulin-like growth factor-1 in activated human T lymphocytes. Journal of Leukocyte Biology, 70(2), 297-305.
Brocardo, M. G. ; Schillaci, R. ; Galeano, A. ; Radrizzani, M. ; White, V. ; González Guerrico, A. ; Santa-Coloma, T. A. ; Roldán, A. / Early effects of insulin-like growth factor-1 in activated human T lymphocytes. In: Journal of Leukocyte Biology. 2001 ; Vol. 70, No. 2. pp. 297-305.
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Brocardo, MG, Schillaci, R, Galeano, A, Radrizzani, M, White, V, González Guerrico, A, Santa-Coloma, TA & Roldán, A 2001, 'Early effects of insulin-like growth factor-1 in activated human T lymphocytes', Journal of Leukocyte Biology, vol. 70, no. 2, pp. 297-305.

Early effects of insulin-like growth factor-1 in activated human T lymphocytes. / Brocardo, M. G.; Schillaci, R.; Galeano, A.; Radrizzani, M.; White, V.; González Guerrico, A.; Santa-Coloma, T. A.; Roldán, A.

In: Journal of Leukocyte Biology, Vol. 70, No. 2, 2001, p. 297-305.

Research output: Contribution to journalArticleResearchpeer-review

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Brocardo MG, Schillaci R, Galeano A, Radrizzani M, White V, González Guerrico A et al. Early effects of insulin-like growth factor-1 in activated human T lymphocytes. Journal of Leukocyte Biology. 2001;70(2):297-305.