Early effects of insulin-like growth factor-1 in activated human T lymphocytes

M. G. Brocardo, R. Schillaci, A. Galeano, M. Radrizzani, V. White, A. González Guerrico, T. A. Santa-Coloma, A. Roldán*

*Corresponding author for this work

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This study evaluates the effects of insulin-like growth factor (IGF)-1 receptor (IGF-1R) down-regulation in stimulated T lymphocytes by investigating the expression of early activation proteins CD69, CD25, and interleukin (IL)-2. We found that IGF-1 does not modify CD69 expression but increases transcription and protein synthesis of CD25 and IL-2. The lowest level of IGF-1R detected after 15 min of activation suggested that the effects of IGF-1 occur at the initiation of cell activation. The activation of IGF-1R was confirmed by IGF-1R phosphorylation and increased phosphorylation of microtubule-associated protein kinase. We also detected the alternative IGF-1 transcripts Ea, with paracrine/autocrine regulation, and Eb, with endocrine regulation, in Jurkat cells and in quiescent T lymphocytes, and we detected IGF-1 protein in the culture medium after stimulation. These data suggest that the proliferative effects of IGF-1 on T lymphocytes include both autocrine/paracrine and endocrine processes.

Original languageEnglish
Pages (from-to)297-305
Number of pages9
JournalJournal of Leukocyte Biology
Volume70
Issue number2
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • CD25
  • IGF-1 mRNA
  • IGF-1 receptor
  • IL-2
  • MAPK

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    Brocardo, M. G., Schillaci, R., Galeano, A., Radrizzani, M., White, V., González Guerrico, A., ... Roldán, A. (2001). Early effects of insulin-like growth factor-1 in activated human T lymphocytes. Journal of Leukocyte Biology, 70(2), 297-305.