TY - JOUR
T1 - EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma
AU - Flores, Isadora L.
AU - Kawahara Sakuma, Rebeca
AU - Miguel, Márcia C. C.
AU - Granato, Daniela C.
AU - Domingues, Romênia R.
AU - Macedo, Carolina C. S.
AU - Carnielli, Carolina M.
AU - Yokoo, Sami
AU - Rodrigues, Priscila C.
AU - Monteiro, Bárbara V. B.
AU - Oliveira, Carine E.
AU - Salmon, Cristiane R.
AU - Nociti, Francisco H.
AU - Lopes, Márcio A.
AU - Santos-Silva, Alan
AU - Winck, Flavia V.
AU - Coletta, Ricardo D.
AU - Paes Leme, Adriana F.
PY - 2016/5
Y1 - 2016/5
N2 - EEF1D (eukaryotic translation elongation factor 1δ) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial–mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT.
AB - EEF1D (eukaryotic translation elongation factor 1δ) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial–mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT.
KW - epithelial-mesenchymal transition
KW - eukaryotic translation elongation factor 1δ
KW - oral cancer
UR - http://www.scopus.com/inward/record.url?scp=84974559465&partnerID=8YFLogxK
U2 - 10.1042/CS20150646
DO - 10.1042/CS20150646
M3 - Article
C2 - 26823560
VL - 130
SP - 785
EP - 799
JO - Clinical Science
JF - Clinical Science
SN - 0143-5221
IS - 10
ER -