EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma

Isadora L. Flores, Rebeca Kawahara Sakuma, Márcia C. C. Miguel, Daniela C. Granato, Romênia R. Domingues, Carolina C. S. Macedo, Carolina M. Carnielli, Sami Yokoo, Priscila C. Rodrigues, Bárbara V. B. Monteiro, Carine E. Oliveira, Cristiane R. Salmon, Francisco H. Nociti, Márcio A. Lopes, Alan Santos-Silva, Flavia V. Winck, Ricardo D. Coletta, Adriana F. Paes Leme

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

EEF1D (eukaryotic translation elongation factor 1δ) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial–mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT.
Original languageEnglish
Pages (from-to)785-799
Number of pages15
JournalClinical Science
Volume130
Issue number10
DOIs
Publication statusPublished - May 2016
Externally publishedYes

Keywords

  • epithelial-mesenchymal transition
  • eukaryotic translation elongation factor 1δ
  • oral cancer

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