EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma

Isadora L. Flores; Rebeca Kawahara Sakuma; Márcia C. C. Miguel; Daniela C. Granato; Romênia R. Domingues; Carolina C. S. Macedo; Carolina M. Carnielli; Sami Yokoo; Priscila C. Rodrigues; Bárbara V. B. Monteiro; Carine E. Oliveira; Cristiane R. Salmon; Francisco H. Nociti; Márcio A.  Lopes; Alan Santos-Silva; Flavia V. Winck; Ricardo D. Coletta; Adriana F. Paes Leme

doi:10.1042/CS20150646

EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma

Isadora L. Flores, Rebeca Kawahara Sakuma, Márcia C. C. Miguel, Daniela C. Granato, Romênia R. Domingues, Carolina C. S. Macedo, Carolina M. Carnielli, Sami Yokoo, Priscila C. Rodrigues, Bárbara V. B. Monteiro, Carine E. Oliveira, Cristiane R. Salmon, Francisco H. Nociti, Márcio A. Lopes, Alan Santos-Silva, Flavia V. Winck, Ricardo D. Coletta, Adriana F. Paes Leme

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

EEF1D (eukaryotic translation elongation factor 1δ) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial–mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT.

Original language	English
Pages (from-to)	785-799
Number of pages	15
Journal	Clinical Science
Volume	130
Issue number	10
DOIs	https://doi.org/10.1042/CS20150646
Publication status	Published - May 2016
Externally published	Yes

Keywords

epithelial-mesenchymal transition
eukaryotic translation elongation factor 1δ
oral cancer

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10.1042/CS20150646

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Flores, I. L., Kawahara Sakuma, R., Miguel, M. C. C., Granato, D. C., Domingues, R. R., Macedo, C. C. S., Carnielli, C. M., Yokoo, S., Rodrigues, P. C., Monteiro, B. V. B., Oliveira, C. E., Salmon, C. R., Nociti, F. H., Lopes, M. A., Santos-Silva, A., Winck, F. V., Coletta, R. D., & Paes Leme, A. F. (2016). EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma. Clinical Science, 130(10), 785-799. https://doi.org/10.1042/CS20150646

Flores, Isadora L. ; Kawahara Sakuma, Rebeca ; Miguel, Márcia C. C. ; Granato, Daniela C. ; Domingues, Romênia R. ; Macedo, Carolina C. S. ; Carnielli, Carolina M. ; Yokoo, Sami ; Rodrigues, Priscila C. ; Monteiro, Bárbara V. B. ; Oliveira, Carine E. ; Salmon, Cristiane R. ; Nociti, Francisco H. ; Lopes, Márcio A. ; Santos-Silva, Alan ; Winck, Flavia V. ; Coletta, Ricardo D. ; Paes Leme, Adriana F. / EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma. In: Clinical Science. 2016 ; Vol. 130, No. 10. pp. 785-799.

@article{34373fb4d2f8413e9b7832eed53d337c,

title = "EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma",

abstract = "EEF1D (eukaryotic translation elongation factor 1δ) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial–mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT.",

keywords = "epithelial-mesenchymal transition, eukaryotic translation elongation factor 1δ, oral cancer",

author = "Flores, {Isadora L.} and {Kawahara Sakuma}, Rebeca and Miguel, {M{\'a}rcia C. C.} and Granato, {Daniela C.} and Domingues, {Rom{\^e}nia R.} and Macedo, {Carolina C. S.} and Carnielli, {Carolina M.} and Sami Yokoo and Rodrigues, {Priscila C.} and Monteiro, {B{\'a}rbara V. B.} and Oliveira, {Carine E.} and Salmon, {Cristiane R.} and Nociti, {Francisco H.} and Lopes, {M{\'a}rcio A.} and Alan Santos-Silva and Winck, {Flavia V.} and Coletta, {Ricardo D.} and {Paes Leme}, {Adriana F.}",

year = "2016",

month = may,

doi = "10.1042/CS20150646",

language = "English",

volume = "130",

pages = "785--799",

journal = "Clinical Science",

issn = "0143-5221",

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Flores, IL, Kawahara Sakuma, R, Miguel, MCC, Granato, DC, Domingues, RR, Macedo, CCS, Carnielli, CM, Yokoo, S, Rodrigues, PC, Monteiro, BVB, Oliveira, CE, Salmon, CR, Nociti, FH, Lopes, MA, Santos-Silva, A, Winck, FV, Coletta, RD & Paes Leme, AF 2016, 'EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma', Clinical Science, vol. 130, no. 10, pp. 785-799. https://doi.org/10.1042/CS20150646

EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma. / Flores, Isadora L.; Kawahara Sakuma, Rebeca; Miguel, Márcia C. C.; Granato, Daniela C.; Domingues, Romênia R.; Macedo, Carolina C. S.; Carnielli, Carolina M.; Yokoo, Sami; Rodrigues, Priscila C.; Monteiro, Bárbara V. B.; Oliveira, Carine E.; Salmon, Cristiane R.; Nociti, Francisco H.; Lopes, Márcio A. ; Santos-Silva, Alan; Winck, Flavia V.; Coletta, Ricardo D.; Paes Leme, Adriana F.

In: Clinical Science, Vol. 130, No. 10, 05.2016, p. 785-799.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma

AU - Flores, Isadora L.

AU - Kawahara Sakuma, Rebeca

AU - Miguel, Márcia C. C.

AU - Granato, Daniela C.

AU - Domingues, Romênia R.

AU - Macedo, Carolina C. S.

AU - Carnielli, Carolina M.

AU - Yokoo, Sami

AU - Rodrigues, Priscila C.

AU - Monteiro, Bárbara V. B.

AU - Oliveira, Carine E.

AU - Salmon, Cristiane R.

AU - Nociti, Francisco H.

AU - Lopes, Márcio A.

AU - Santos-Silva, Alan

AU - Winck, Flavia V.

AU - Coletta, Ricardo D.

AU - Paes Leme, Adriana F.

PY - 2016/5

Y1 - 2016/5

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AB - EEF1D (eukaryotic translation elongation factor 1δ) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial–mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT.

KW - epithelial-mesenchymal transition

KW - eukaryotic translation elongation factor 1δ

KW - oral cancer

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U2 - 10.1042/CS20150646

DO - 10.1042/CS20150646

M3 - Article

C2 - 26823560

VL - 130

SP - 785

EP - 799

JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

IS - 10

ER -

EEF1D modulates proliferation and epithelial-mesenchymal transition in oral squamous cell carcinoma

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Keywords

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