Effect of amino acid peroxides on the erythrocyte

Miroslaw Soszyński*, Anna Filipiak, Grzegorz Bartosz, Janusz M. Gebicki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

The effect of amino acid peroxides, relatively stable products of irradiation of amino acid solutions, on erythrocyte componentswas studied. Interaction of proline, lysine, valine, and leucine peroxides (100-300 μM) with erythrocyte membranes brought about a decrease of membrane protein SH group content and of activities of (Na +, K +)-ATPase and Ca 2+-ATPase, and induced aggregation of membrane proteins, due mainly to the formation of interpeptide disulfides. Interaction of amino acid peroxides with hemoglobin brought about hemoglobin oxidation to methemoglobin. The effects of amino acid peroxides are similar to those of t-butyl hydroperoxide. These results indicate that peroxides of amino acid and proteins, which can also be formed under physiological conditions, may be mediators of the cellular action of reactive oxygen species.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalFree Radical Biology and Medicine
Volume20
Issue number1
DOIs
Publication statusPublished - 1996

Keywords

  • ATPase
  • Erythrocyte
  • Erythrocyte membrane
  • Free radicals
  • Hemoglobin
  • Peroxides
  • Thiol groups

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