Abstract
The effect of amino acid peroxides, relatively stable products of irradiation of amino acid solutions, on erythrocyte componentswas studied. Interaction of proline, lysine, valine, and leucine peroxides (100-300 μM) with erythrocyte membranes brought about a decrease of membrane protein SH group content and of activities of (Na +, K +)-ATPase and Ca 2+-ATPase, and induced aggregation of membrane proteins, due mainly to the formation of interpeptide disulfides. Interaction of amino acid peroxides with hemoglobin brought about hemoglobin oxidation to methemoglobin. The effects of amino acid peroxides are similar to those of t-butyl hydroperoxide. These results indicate that peroxides of amino acid and proteins, which can also be formed under physiological conditions, may be mediators of the cellular action of reactive oxygen species.
Original language | English |
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Pages (from-to) | 45-51 |
Number of pages | 7 |
Journal | Free Radical Biology and Medicine |
Volume | 20 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1996 |
Keywords
- ATPase
- Erythrocyte
- Erythrocyte membrane
- Free radicals
- Hemoglobin
- Peroxides
- Thiol groups