Effect of anaesthetic and choice of neuromuscular blocker on vagal control of heart rate under laboratory animal experimental conditions

Divya Sarma Kandukuri, Jacqueline K. Phillips, Mark Tahmindjis, Cara M. Hildreth

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Neuromuscular-blocking agents are commonly used in laboratory animal research settings. Due to actions of cholinergic receptors at locations other than the motor end-plate, these agents have a strong propensity to modulate autonomic outflow and may therefore not be desirable in studies examining autonomic function. This study aimed to compare the effect of two non-depolarizing neuromuscular-blocking agents, pancuronium and cisatracurium, on blood pressure, heart rate and non-invasive indices of autonomic function (heart rate variability, systolic blood pressure variability and baroreflex sensitivity) under two different types of anaesthesia in Lewis rats. Pancuronium produced a profound vagolytic response characterized by tachycardia, reduction in heart rate variability and baroreflex sensitivity under urethane anaesthesia, and with minimal effect under isoflurane anaesthesia. Conversely, cisatracurium produced no evidence of vagolytic action under either urethane or isoflurane anaesthesia. Therefore, for studies interested in examining autonomic function, particularly baroreflex or vagal function, neuromuscular blockade would be best achieved using cisatracurium.

LanguageEnglish
Pages280-291
Number of pages12
JournalLaboratory Animals
Volume52
Issue number3
Early online date1 Sep 2017
DOIs
Publication statusPublished - Jun 2018

Fingerprint

Neuromuscular Blocking Agents
Laboratory Animals
anesthetics
laboratory animals
Anesthetics
Baroreflex
heart rate
anesthesia
Anesthesia
Heart Rate
Pancuronium
urethane
Isoflurane
Urethane
isoflurane
Blood Pressure
Motor Endplate
Neuromuscular Blockade
animal research
cholinergic receptors

Keywords

  • baroreceptor reflex
  • heart rate
  • neuromuscular blockade
  • parasympathetic nervous system

Cite this

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abstract = "Neuromuscular-blocking agents are commonly used in laboratory animal research settings. Due to actions of cholinergic receptors at locations other than the motor end-plate, these agents have a strong propensity to modulate autonomic outflow and may therefore not be desirable in studies examining autonomic function. This study aimed to compare the effect of two non-depolarizing neuromuscular-blocking agents, pancuronium and cisatracurium, on blood pressure, heart rate and non-invasive indices of autonomic function (heart rate variability, systolic blood pressure variability and baroreflex sensitivity) under two different types of anaesthesia in Lewis rats. Pancuronium produced a profound vagolytic response characterized by tachycardia, reduction in heart rate variability and baroreflex sensitivity under urethane anaesthesia, and with minimal effect under isoflurane anaesthesia. Conversely, cisatracurium produced no evidence of vagolytic action under either urethane or isoflurane anaesthesia. Therefore, for studies interested in examining autonomic function, particularly baroreflex or vagal function, neuromuscular blockade would be best achieved using cisatracurium.",
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Effect of anaesthetic and choice of neuromuscular blocker on vagal control of heart rate under laboratory animal experimental conditions. / Kandukuri, Divya Sarma; Phillips, Jacqueline K.; Tahmindjis, Mark; Hildreth, Cara M.

In: Laboratory Animals, Vol. 52, No. 3, 06.2018, p. 280-291.

Research output: Contribution to journalArticleResearchpeer-review

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